Kudryasheva N S, Nemtseva E V, Sizykh A G, Kratasyuk V A, Visser A J W G
Institute of Biophysics, Siberian Branch of the Russian Academy of Sciences, Akademgorodok, Krasnoyarsk 660036, Russia.
J Photochem Photobiol B. 2002 Nov;68(2-3):88-92. doi: 10.1016/s1011-1344(02)00360-3.
The hypothesis of activity of the upper electron-excited states of the bacterial bioluminescent emitter was verified using dye molecules as foreign energy acceptors. Six compounds were selected having fluorescent state energies ranging from 25,700 to 32,000 cm(-1) (anthracene, pyrene, 1.4-bis(5-phenyloxasol-2-yl)benzene (POPOP), p-bis(o-methylstyryl)benzene (MSB), 2-methoxy-naphtalene, p-terphenyl), exceeding that of the bioluminescent emitter (22,000 cm(-1)). Their absorption spectra do not overlap with the bioluminescence spectrum; the trivial light absorption and the intermolecular resonance S-S energy transfer were excluded. Bacterial bioluminescent spectra of the coupled enzyme system NADH:FMN-oxidoreductase-luciferase in the presence of MSB were presented as an example. The weak sensitized fluorescence of MSB was registered. The results obtained have confirmed the activity of the energetic precursor in the bacterial bioluminescence. Its energy can be located in the interval of 26,000-27,000 cm(-1).
利用染料分子作为外来能量受体，验证了细菌生物发光发射体上电子激发态的活性假说。选择了六种化合物，其荧光态能量范围为25,700至32,000厘米⁻¹（蒽、芘、1,4 - 双（5 - 苯基恶唑 - 2 - 基）苯（POPOP）、对 - 双（邻 - 甲基苯乙烯基）苯（MSB）、2 - 甲氧基萘、对三联苯），超过了生物发光发射体的能量（22,000厘米⁻¹）。它们的吸收光谱与生物发光光谱不重叠；排除了平凡的光吸收和分子间共振S - S能量转移。以MSB存在下的偶联酶系统NADH：FMN - 氧化还原酶 - 荧光素酶的细菌生物发光光谱为例。记录到了MSB微弱的敏化荧光。所得结果证实了细菌生物发光中高能前体的活性。其能量可位于26,000 - 27,000厘米⁻¹区间。
