Drake Pascal M W, Chargelegue Daniel, Vine Nicholas D, Van Dolleweerd Craig J, Obregon Patricia, Ma Julian K-C
Department of Oral Medicine and Pathology, Unit of Immunology, Guy's Tower, Guy's Hospital, GKT Dental Institute, London Bridge, London SE1 9RT, UK.
FASEB J. 2002 Dec;16(14):1855-60. doi: 10.1096/fj.02-0148com.
The feasibility of using antibody expressing transgenic plants either to neutralize bioactive molecules in the rhizosphere, or to accumulate and concentrate the molecules in leaves has been demonstrated in a model system consisting of hydroponic Nicotiana plant cultures expressing a murine monoclonal IgG1. Two transgenic plant types were used; in the first, functional antibody was rhizosecreted and shown to bind with antigen in the surrounding medium to form an immune complex. In the second, a transmembrane sequence retained monoclonal antibody in the plants, on the plasma membrane. Antigen added to the nutrient medium around the roots of mIgG plants was transported within 24 h to the topmost leaves of the plant where it was sequestered as an immune complex by binding to antibody on the cell membrane. Concentration of immune complex in the leaf tissue remained constant over a 72 h period after removal of antigen from nutrient medium. Free antigen was not detected in the leaves of wild-type plants. The two strategies of rhizosecretion-mediated binding and sequestration in leaf tissue could potentially be used in the phytoremediation of any pollutant for which it is possible to generate a monoclonal antibody.
在一个由表达鼠单克隆IgG1的水培烟草植物培养物组成的模型系统中，已证明利用表达抗体的转基因植物来中和根际中的生物活性分子，或在叶片中积累和浓缩这些分子的可行性。使用了两种转基因植物类型；在第一种中，功能性抗体被根部分泌，并显示与周围培养基中的抗原结合形成免疫复合物。在第二种中，一个跨膜序列将单克隆抗体保留在植物的质膜上。添加到表达mIgG植物根部周围营养培养基中的抗原在24小时内被转运到植物最顶端的叶片，在那里它通过与细胞膜上的抗体结合而作为免疫复合物被隔离。从营养培养基中去除抗原后，叶组织中免疫复合物的浓度在72小时内保持恒定。在野生型植物的叶片中未检测到游离抗原。根部分泌介导的结合和叶组织中隔离这两种策略有可能用于对任何能够产生单克隆抗体的污染物进行植物修复。
