Monoclonal antibody against free beta-subunit of human chorionic gonadotropin.

作者信息

Baluja Conde Ileana B, Brito Moreno Adys I, Acosta Bas Carmen, Zalueta Rodríguez Orlando, Melchor Rodríguez Antonio, Amores Sanchéz Isis, Martínez Luisa, Hernández Milenen

机构信息

Laboratorio de Anticuerpos Monoclonales, Centro de Inmunoensayo, Apartado 6653, Ave 25 y 134, Cubanacán, Playa, Ciudad Habana, Cuba.

出版信息

Hybrid Hybridomics. 2002 Oct;21(5):381-4. doi: 10.1089/153685902761022733.

DOI:10.1089/153685902761022733

PMID:12470481

Abstract

Spleen cells from BALB/c mice immunized with free native human chorionic gonadotropin hormone beta-subunit (beta hCG) were fused with mouse myeloma cells (P3/X63-Ag8) and one hybridoma secreting monoclonal antibodies (MAbs), was obtained. This hybridoma specifically recognizes beta hCG and does not cross-react with other human glycoprotein hormones, such as luteinizing hormone (LH), follicle-stimulating hormone (FSH), thyroid-stimulating hormone (TSH), and human chorionic gonadotropin (hCG). The MAb was of the IgG(1) subclass and ascitic fluid from this hybridoma was purified by affinity chromatography on Protein A-Sepharose CL-4B column to isolate the IgG(1) active fraction. The affinity constant of this MAb was 1.5 x 10(10)M(-1).

摘要