核糖核酸酶E参与α-变形菌纲中5'-端23S核糖体RNA的加工过程。

RNase E is involved in 5'-end 23S rRNA processing in alpha-Proteobacteria.

作者信息

Klein Franziska, Evguenieva-Hackenberg Elena

机构信息

Institut für Mikrobiologie und Molekularbiologie der Justus-Liebig-Universität Giessen, Heinrich-Buff-Ring 26-32, 35392 Giessen, Germany.

出版信息

Biochem Biophys Res Commun. 2002 Dec 20;299(5):780-6. doi: 10.1016/s0006-291x(02)02738-9.

DOI:10.1016/s0006-291x(02)02738-9

PMID:12470646

Abstract

In Rhodobacter capsulatus and Rhizobium leguminosarum, an internal transcribed spacer consisting of helices 9 and 10 is removed during 23S rRNA processing, which leads to the occurrence of a 5.8S-like rRNA. The particular rRNA maturation steps are not known, with exception of the initial RNase III cleavage in helix 9. We found that GC-rich stem-loop structures of helix 9, which are released by RNase III, are immediately degraded. The degradation of helix 10 is slower and its kinetics differs in both species. Nevertheless, the helix 10 processing mechanism is conserved and includes cleavages by RNase E.

摘要

在荚膜红细菌和豌豆根瘤菌中，由螺旋9和螺旋10组成的内部转录间隔区在23S rRNA加工过程中被去除，这导致了类似5.8S rRNA的出现。除了螺旋9中最初的核糖核酸酶III切割外，特定的rRNA成熟步骤尚不清楚。我们发现，核糖核酸酶III释放的富含GC的螺旋9茎环结构会立即降解。螺旋10的降解较慢，其动力学在这两个物种中有所不同。然而，螺旋10的加工机制是保守的，包括核糖核酸酶E的切割。