Agami Reuven
Division of Tumor Biology and Center for Biomedical Genetics, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands.
Curr Opin Chem Biol. 2002 Dec;6(6):829-34. doi: 10.1016/s1367-5931(02)00378-2.
Introduction of double-stranded RNAs into cells can suppress gene expression by mechanisms such as mRNA degradation or inhibition of translation. In mammalian cells, these two responses intersect, a feature that was recently used for the development of novel tools for stable and specific gene inactivation. These new tools were successfully applied to inhibit tumorigenicity and viral replication. Future development of appropriate in vivo delivery systems may make this technology useful for disease therapy.
将双链RNA导入细胞可以通过mRNA降解或翻译抑制等机制来抑制基因表达。在哺乳动物细胞中，这两种反应相互交织，这一特性最近被用于开发稳定且特异性基因失活的新型工具。这些新工具已成功应用于抑制肿瘤发生和病毒复制。未来开发合适的体内递送系统可能会使这项技术用于疾病治疗。
