Ku S K, Lee H S, Lee J H
Pharmacology & Toxicology Laboratory, Central Research Laboratories, Dong-Wha Pharm. Ind. Co.
Eur J Histochem. 2002;46(3):229-36. doi: 10.4081/1684.
The regional distribution and frequency of the pancreatic endocrine cells in the SKH-1 hairless mouse were studied by an immunohistochemical (peroxidase anti-peroxidase; PAP) method using four types of specific antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). The pancreas of mice were divided into three portions; pancreatic islets, exocrine and pancreatic ducts. The pancreatic islets were further subdivided into three regions (central, mantle and peripheral region) according to their located types of immunoreactive cells. In the pancreatic islet portions, insulin-immunoreactive cells were located in the central and mantle regions with 84.60 +/- 7.65 and 33.00 +/- 12.45/100 cells frequencies, respectively, but most of somatostatin-, glucagon- and PP-immunoreactive cells were detected in the mantle and peripheral regions. In the mantle region, somatostatin-, glucagon- and PP-immunoreactive cells were demonstrated with 28.70 +/- 9.91, 52.00 +/- 14.05 and 2.60 +/- 1.51/100 cells frequencies, respectively, and showed 6.20 +/- 2.86, 15.30 +/- 5.31 and 21.50 +/- 10.28/100 cells frequencies, respectively in peripheral regions. However, glucagon-immunoreactive cells were also demonstrated in the central regions with 4.00 +/- 2.83/100 cells frequency. In the exocrine portions, insulin-, glucagon-, somatostatin- and PP-immunoreactive cells were demonstrated in the SKH-1 mouse with 0.90 +/- 0.74, 0.80 +/- 0.79,4.90 +/- 3.54 and 2.70 +/- 1.34/100 cells frequencies, respectively. In the pancreatic duct portions, insulin-, glucagon- and somatostatin-immunoreactive cells were demonstrated in the subepithelial connective tissues and showed islet-like appearances with 30.30 +/- 14.67, 2.70 +/- 3.13 and 5.90 +/- 4.23/100 cells frequencies, respectively. However, no PP-immunoreactive cells were demonstrated in these regions. In conclusion, some peculiar distributional patterns of pancreatic endocrine cells were found in the SKH-1 hairless mouse.
采用免疫组织化学（过氧化物酶抗过氧化物酶；PAP）方法，使用针对胰岛素、胰高血糖素、生长抑素和人胰多肽（PP）的四种特异性抗血清，研究了SKH - 1无毛小鼠胰腺内分泌细胞的区域分布和频率。小鼠的胰腺分为三个部分：胰岛、外分泌部和胰管。胰岛根据其免疫反应性细胞的定位类型进一步细分为三个区域（中央、被膜和周边区域）。在胰岛部分，胰岛素免疫反应性细胞分别位于中央和被膜区域，频率分别为84.60±7.65和33.00±12.45/100个细胞，但大多数生长抑素、胰高血糖素和PP免疫反应性细胞在被膜和周边区域被检测到。在被膜区域，生长抑素、胰高血糖素和PP免疫反应性细胞的频率分别为28.70±9.91、52.00±14.05和2.60±1.51/100个细胞，在周边区域分别为6.20±2.86、15.30±5.31和21.50±10.28/100个细胞。然而，胰高血糖素免疫反应性细胞在中央区域也有显示，频率为4.00±2.83/100个细胞。在外分泌部分，SKH - 1小鼠中胰岛素、胰高血糖素、生长抑素和PP免疫反应性细胞的频率分别为0.90±0.74、0.80±0.79、4.90±3.54和2.70±1.34/100个细胞。在胰管部分，胰岛素、胰高血糖素和生长抑素免疫反应性细胞在上皮下结缔组织中显示，呈胰岛样外观，频率分别为30.30±14.67、2.70±3.13和5.90±4.23/100个细胞。然而，在这些区域未发现PP免疫反应性细胞。总之，在SKH - 1无毛小鼠中发现了胰腺内分泌细胞的一些特殊分布模式。
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