Banks Charmian, Bateman Adrian, Payne Richard, Johnson Penny, Sheron Nick
Southampton General Hospital, Southampton, UK.
J Pathol. 2003 Jan;199(1):28-35. doi: 10.1002/path.1245.
Mucosal changes in inflammatory bowel disease (IBD) are characterized by ulcerative lesions accompanied by prominent cellular infiltrates in the bowel wall. Chemokines are chemotactic cytokines that are able to promote leukocyte migration to areas of inflammation and are also able to initiate cell activation events. They have recently been implicated in the pathophysiology of many disease states. The aim of this study was to detail the degree and distribution of specific chemokines, interleukin (IL)-8, monocyte chemoattractant protein (MCP)-1, -2, and -3, and macrophage inflammatory protein (MIP)-1alpha and -1beta, in IBD mucosa. Thirty-nine patients were included, ten controls, 20 ulcerative colitis (UC), and nine Crohn's disease (CD), with a range of disease activity. Colonic mucosal biopsies were collected from UC, CD, and control patients and embedded in glycol methacrylate. Two-micrometre-thick sections were cut and stained using immunohistochemistry for chemokine protein expression. Sections were analysed using a light microscope. Expression of all types of chemokine protein was detected in colonic mucosa from both control and IBD patients. Patterns of staining between IBD patients and controls differed significantly, but CD and UC patients demonstrated similar patterns of staining. Individual chemokine expression was found to be significantly up-regulated in IBD when patients were compared with the non-diseased group in all areas of the mucosal sections. Up-regulated chemokine expression correlated with increasing activity of the disease. It is concluded that human colonic chemokine expression is non-selectively up-regulated in IBD. The results supported the hypothesis that the degree of local inflammation and tissue damage in UC and CD is dependent on local expression of specific chemokines within IBD tissues.
炎症性肠病（IBD）的黏膜变化特征为溃疡性病变，并伴有肠壁内明显的细胞浸润。趋化因子是一类趋化性细胞因子，能够促进白细胞迁移至炎症区域，还能启动细胞激活事件。它们最近被认为与许多疾病状态的病理生理学有关。本研究的目的是详细阐述特定趋化因子、白细胞介素（IL）-8、单核细胞趋化蛋白（MCP）-1、-2和-3以及巨噬细胞炎性蛋白（MIP）-1α和-1β在IBD黏膜中的程度和分布。纳入了39例患者，包括10例对照、20例溃疡性结肠炎（UC）和9例克罗恩病（CD），疾病活动程度各异。从UC、CD和对照患者中采集结肠黏膜活检组织，并包埋于甲基丙烯酸乙二醇酯中。切成2微米厚的切片，采用免疫组织化学法对趋化因子蛋白表达进行染色。切片用光学显微镜进行分析。在对照和IBD患者的结肠黏膜中均检测到所有类型趋化因子蛋白的表达。IBD患者与对照之间的染色模式存在显著差异，但CD和UC患者的染色模式相似。与非患病组相比，在黏膜切片的所有区域中，IBD患者个体趋化因子表达均显著上调。趋化因子表达上调与疾病活动度增加相关。得出的结论是，IBD中人类结肠趋化因子表达非选择性上调。结果支持了UC和CD中局部炎症和组织损伤程度取决于IBD组织内特定趋化因子局部表达的假说。
