Ts'ao C H, Green D, Schultz K
Br J Haematol. 1976 Feb;32(2):225-33. doi: 10.1111/j.1365-2141.1976.tb00925.x.
We have investigated platelet morphology and function in human maternal-newborn pairs. Fibrinogen concentration and factor-VIII activity in plasma were also determined. Our results showed that, compared to maternal platelets, neonatal platelets were poorly responsive to adenosine diphosphate, adrenaline and collagen. Uptake of labelled serotonin by neonatal and maternal platelets was the same, but release of the radioactivity was reduced in the former. Phagocytic activity of neonatal platelets, demonstrated with latex particles, was similar to that of maternal platelets. Although the ultrastructure of neonatal platelets approximated that of maternal platelets, immature appearing platelets were occasionally found in the neonatal samples. An unanticipated finding was that platelet aggregation induced by ristocetin was more vigorous in neonatal than in maternal platelet-rich plasma samples. Furthermore, neonal -lasma, which had lower fibrinogen and factor-VIII content than maternal plasma, facilitated maternal platelet aggregation by ristocetin and showed a greater ability than maternal plasma to promote ristocetin-induced aggregation of platelets of a patient with von Willebrand's disease. These results indicate that the plasma of neonates contains large quantities of the ristocetin-dependent platelet aggregation factor (RAF), probably more than is in maternal plasma, despite the higher levels of factor-VIII procoagulant activity in the latter. Thus, in the newborn, there is a clear dissociation between factor-VIII clotting activity and the RAF activity.
我们研究了人类母婴配对中血小板的形态和功能。还测定了血浆中的纤维蛋白原浓度和因子VIII活性。我们的结果表明，与母体血小板相比，新生儿血小板对二磷酸腺苷、肾上腺素和胶原的反应较差。新生儿和母体血小板对标记血清素的摄取相同，但前者的放射性释放减少。用乳胶颗粒证明的新生儿血小板吞噬活性与母体血小板相似。尽管新生儿血小板的超微结构与母体血小板相似，但在新生儿样本中偶尔会发现外观不成熟的血小板。一个意外发现是，在富含新生儿血小板的血浆样本中，瑞斯托菌素诱导的血小板聚集比母体样本中更强烈。此外，新生儿血浆中纤维蛋白原和因子VIII含量低于母体血浆，它能促进瑞斯托菌素诱导的母体血小板聚集，并且比母体血浆更能促进血管性血友病患者血小板的瑞斯托菌素诱导聚集。这些结果表明，尽管母体血浆中因子VIII促凝血活性较高，但新生儿血浆中含有大量瑞斯托菌素依赖性血小板聚集因子（RAF），可能比母体血浆中的更多。因此，在新生儿中，因子VIII凝血活性与RAF活性之间存在明显的分离。
