[六甲撑双乙酰胺对人黏液表皮样癌细胞系MEC-1凋亡及细胞增殖相关基因表达的影响]
[Effects of hexamethylene bisacetamide on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1].
作者信息
Wang Lijing, Wang Jing
机构信息
Department of Stomatology, Lanzhou Medical College, Lanzhou 730000, China.
出版信息
Zhonghua Kou Qiang Yi Xue Za Zhi. 2002 Mar;37(2):120-2.
OBJECTIVE
To investigate the effects of hexamethylene bisacetamide (HMBA) on related genes expression of apoptosis and cell proliferating in human mucoepidermoid carcinoma cell line MEC-1.
METHODS
MEC-1 was induced 72 hours by 0.002 mol/L HMBA in vitro. The morphologic observation and the expression of PCNA, bcl-2, bax protein and bcl-2 mRNA were determined by microscope, immunohistochemistry, in situ hybridization and image analysis.
RESULTS
The expression of bax, bcl-2 protein and bcl-2 mRNA were higher than control group (P < 0.01). The related genes of cell proliferating PCNA were decreased in control group.
CONCLUSIONS
HMBA could inhibit the MEC-1 cell proliferation by down-regulation of PCNA expression, it could also induce apoptosis effectively that might be through the way of up-regulation of bax and bcl-2 gene expression.
目的
研究六甲撑双乙酰胺（HMBA）对人黏液表皮样癌细胞系MEC-1凋亡相关基因表达及细胞增殖的影响。
方法
体外以0.002mol/L HMBA诱导MEC-1细胞72小时。通过显微镜、免疫组织化学、原位杂交及图像分析观察细胞形态，检测增殖细胞核抗原（PCNA）、bcl-2、bax蛋白及bcl-2 mRNA的表达。
结果
bax、bcl-2蛋白及bcl-2 mRNA的表达高于对照组（P<0.01）。对照组中细胞增殖相关基因PCNA表达降低。
结论
HMBA可通过下调PCNA表达抑制MEC-1细胞增殖，还可通过上调bax和bcl-2基因表达有效诱导细胞凋亡。
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