肿瘤坏死因子-α mRNA在小鼠病毒性心肌炎中的表达及其意义

The expression of TNF-alpha mRNA and its significance in murine viral myocarditis.

作者信息

Zhang Song, Rao Bangfu

机构信息

Cardiovascular Department, Zhejiang Provincial Hospital, Hangzhou 310014, China.

出版信息

Zhonghua Bing Li Xue Za Zhi. 2002 Jun;31(3):250-2.

PMID:12475445

Abstract

OBJECTIVE

To study the change of serum tumor necrosis factor-alpha (TNF-alpha) and its mRNA in viral myocarditis (VMC) and its role in VMC.

METHODS

The expression of TNF-alpha mRNA in VMC mice was studied by the way of RT-PCR. Changes of serum TNF-alpha on day 3, 5, 7, 9, 15 and 35 after virus inoculation in VMC mice were assayed using ELISA. The possible relationship of TNF-alpha and VMC was analysed.

RESULTS

The expression of TNF-alpha mRNA in VMC mice increased apparently. The level of TNF-alpha mRNA expressed on the 7th day after virus inoculation (1.94) was higher than that on the 3rd day (1.09). Serum TNF-alpha of the VMC mice was higher than that of the control mice (127.2 +/- 13.8) from the 3rd to 35th day after virus inoculation (3rd day group 159.7 +/- 40.8, 5th day group 212.7 +/- 40.0, 7th day group 296.7 +/- 34.3, 9th day group 267.3 +/- 41.4, 15th day groups 187.8 +/- 49.6, and 35th day groups 155.4 +/- 35.9) and the peak expression was on the 7th day after the inoculation (P < 0.01 compared with the control group).

CONCLUSIONS

Both serum TNF-alpha and its mRNA increased in murine VMC, which may take part in the pathogenesis of VMC.

摘要

目的

研究病毒性心肌炎（VMC）中血清肿瘤坏死因子-α（TNF-α）及其mRNA的变化及其在VMC中的作用。

方法

采用逆转录聚合酶链反应（RT-PCR）法研究VMC小鼠中TNF-α mRNA的表达。用酶联免疫吸附测定法（ELISA）检测VMC小鼠病毒接种后第3、5、7、9、15和35天血清TNF-α的变化。分析TNF-α与VMC之间可能的关系。

结果

VMC小鼠中TNF-α mRNA的表达明显增加。病毒接种后第7天（1.94）表达的TNF-α mRNA水平高于第3天（1.09）。病毒接种后第3至35天，VMC小鼠的血清TNF-α高于对照小鼠（第3天组159.7±40.8，第5天组212.7±40.0，第7天组296.7±34.3，第9天组267.3±41.4，第15天组187.8±49.6，第35天组155.4±35.9），且接种后第7天表达量达到峰值（与对照组相比，P<0.01）。