离体肠上皮细胞中的钠离子转运。II. 主动转运糖对空肠和回肠分离细胞中钠离子外流影响的比较。

Sodium ion transport in isolated intestinal epithelial cells. II. Comparison of the effect of actively transported sugars on sodium ion efflux in cells isolated from jejunum and ileum.

作者信息

Gall D G, Chapman D

出版信息

Biochim Biophys Acta. 1976 Jan 21;419(2):314-9. doi: 10.1016/0005-2736(76)90357-6.

Abstract

Na+ transport studies in intestinal epithelial cells indicate that enterocytes from different regions of the small intestine differ in their response to actively transported sugars. 1. Compared with sugar-free medium total Na+ efflux rate constants from isolated rat jejunal cells were significantly increased when medium contained actively transported sugars, glucose and galactose, but not when medium contained fructose. 2 In contrast total Na+ efflux rate constants from isolated rat ileal cells did not respond to actively transported sugars, glucose and galactose. 3. Similar results for the effect of actively transported sugars on Na+ ellux were obtained for isolated rabbit jejunal and ileal epithelial cells. 4. Passive Na+ efflux rate constants for isolated jejunal and ileal enterocytes are not significantly different, indicating similiar permeability characteristics.

摘要

对肠道上皮细胞中钠离子转运的研究表明，来自小肠不同区域的肠上皮细胞对主动转运糖类的反应存在差异。1. 与无糖培养基相比，当培养基中含有主动转运的糖类（葡萄糖和半乳糖）时，分离出的大鼠空肠细胞的总钠离子外流速率常数显著增加，但当培养基中含有果糖时则不然。2. 相比之下，分离出的大鼠回肠细胞的总钠离子外流速率常数对主动转运的糖类（葡萄糖和半乳糖）没有反应。3. 对于分离出的兔空肠和回肠上皮细胞，主动转运的糖类对钠离子外流的影响也得到了类似结果。4. 分离出的空肠和回肠肠上皮细胞的被动钠离子外流速率常数没有显著差异，表明其通透性特征相似。