Haslett B G, Yarwood A, Evans I M, Boulter D
Biochim Biophys Acta. 1976 Jan 20;420(1):122-32. doi: 10.1016/0005-2795(76)90351-2.
We have measured the molecular weight of the small subunit of Fraction I protein from pea and broad bean by sodium dodecyl sulphate polyacrylamide gel electrophoresis, Sephadex gel-filtration and amino acid composition data. The results suggest a molecular weight of 12 000-14 500, although measurements by gel-filtration in alkali suggest a molecular weight of approximately 22 000. N-terminal amino acid sequence data and C-terminal determinations show that the protein consists of a single type of polypeptide chain, although the anomalously high molecular weight obtained on gel-filtration in alkali does not preclude the existence of the polypeptides as dimers under certain conditions.
我们通过十二烷基硫酸钠聚丙烯酰胺凝胶电泳、葡聚糖凝胶过滤以及氨基酸组成数据，测定了豌豆和蚕豆中I组分蛋白小亚基的分子量。结果表明其分子量为12000 - 14500，尽管在碱性条件下进行凝胶过滤测定的分子量约为22000。N端氨基酸序列数据和C端测定结果表明该蛋白由单一类型的多肽链组成，尽管在碱性条件下凝胶过滤得到的异常高分子量并不排除在某些条件下多肽以二聚体形式存在的可能性。
