大鼠、猫和豚鼠体内内源性钴胺素及其他咕啉类化合物的组织分布。

Tissue distribution of endogenous cobalamins and other corrins in the rat, cat and guinea pig.

Quadros E V, Matthews D M, Wise I J, Linnell J C

Biochim Biophys Acta. 1976 Jan 14;421(1):141-52. doi: 10.1016/0304-4165(76)90178-1.

Abstract

Methylcobalamin, adenosylcobalamin, hydroxocobalamin and cyanocobalamin have been estimated by a chromato-bioautographic techniques in 16 tissues from healthy rats and in five guinea pig tissues. 2. Plasma and erythrocyte cobalamins have been estimated in rats, cats and guinea pigs and the results compared with those in man. 3. Unidentified corrins were detected in 8 of the 16 rat tissues and in 3 of the 5 guinea pig tissues analysed, but were not present in tissues from specific pathogen-free rats nor in the standard laboratory diet. 4. Adenosylcobalamin was the major corrin in 8 of the 16 rat tissues. In the remainder hydroxocobalamin predominated or was present in equal proportions with adenosylcobalamin. Methylcobalamin was detected in the majority of rat tissues but at levels much lower than those in human tissues. Small amounts of cyanocobalamin were detected also and levels were higher than those of methylcobalamin in 8 of the 16 tissues. 5. In the rat, cat and guinea pig, levels of methylcobalamin and hydroxobalamin were higher in erythrocytes than in plasma, a pattern almost the complete reverse of that in man.

摘要

采用色谱生物自显影技术对健康大鼠的16种组织以及豚鼠的5种组织中的甲钴胺、腺苷钴胺、羟钴胺和氰钴胺进行了测定。2. 对大鼠、猫和豚鼠的血浆及红细胞钴胺素进行了测定，并将结果与人的结果进行了比较。3. 在分析的16种大鼠组织中的8种以及5种豚鼠组织中的3种中检测到了未鉴定的咕啉，但在无特定病原体大鼠的组织中以及标准实验室饮食中均未发现。4. 腺苷钴胺是16种大鼠组织中8种组织的主要咕啉。在其余组织中，羟钴胺占主导地位，或与腺苷钴胺含量相当。在大多数大鼠组织中检测到了甲钴胺，但含量远低于人体组织中的水平。还检测到少量氰钴胺，在16种组织中的8种中，其含量高于甲钴胺。5. 在大鼠、猫和豚鼠中，红细胞中的甲钴胺和羟钴胺水平高于血浆，这一模式与人几乎完全相反。