Eyckerman Sven, Lemmens Irma, Lievens Sam, Van der Heyden Jose, Verhee Annick, Vandekerckhove Joël, Tavernier Jan
Flanders Interuniversity Institute for Biotechnology, Department of Medical Protein Research (VIB09), Ghent University, Faculty of Medicine and Health Sciences, A. Baertsoenkaai 3, B-9000 Ghent, Belgium.
Sci STKE. 2002 Dec 10;2002(162):pl18. doi: 10.1126/stke.2002.162.pl18.
Identifying the interaction partners of a protein is a straightforward way to gain insight into the protein's function and to position it in an interaction network such as a signal transduction pathway. Various techniques have been developed to serve this purpose, and some are specifically designed to study posttranslational modifications in mammalian proteins and to clarify their normal physiological context. However, several intrinsic constraints limit the use of these technologies, and most are not suitable for screening for new interacting partners. In the Mammalian Protein-Protein Interaction Trap (MAPPIT) Protocol described here, knowledge of cytokine receptor signaling has been used to design a versatile genetic tool that can be used analytically and for detection of new protein-protein interactions in mammalian cells.
确定蛋白质的相互作用伙伴是深入了解蛋白质功能并将其置于诸如信号转导途径等相互作用网络中的直接方法。已经开发了各种技术来实现这一目的，其中一些技术专门用于研究哺乳动物蛋白质的翻译后修饰并阐明其正常生理背景。然而，一些内在限制阻碍了这些技术的应用，并且大多数技术不适用于筛选新的相互作用伙伴。在此描述的哺乳动物蛋白质-蛋白质相互作用陷阱（MAPPIT）方案中，细胞因子受体信号传导的知识被用于设计一种通用的遗传工具，该工具可用于分析以及检测哺乳动物细胞中的新蛋白质-蛋白质相互作用。
