Serebriiskii Ilya G, Kotova Elena
Division of Basic Science, Fox Chase Cancer Center, Philadelphia, PA, USA.
Methods Mol Biol. 2004;261:263-96. doi: 10.1385/1-59259-762-9:263.
To characterize a protein's function, it is often advantageous to identify other proteins with which it interacts. The yeast two-hybrid system is one of the most versatile methods available for detection and characterization of protein-protein interactions, and in the recent years it has become a mature and robust technology. A further improvement to this technique is the ability to examine and distinguish more than one interaction simultaneously. This is achieved in the Dual Bait, which has successfully been used to detect proteins and peptides that target specific motifs in larger proteins, to facilitate rapid identification of specific interactors from a pool of putative interacting proteins obtained in a library screen, and to score specific drug-mediated disruption of protein-protein interaction.
为了表征一种蛋白质的功能，鉴定与之相互作用的其他蛋白质通常是有益的。酵母双杂交系统是可用于检测和表征蛋白质-蛋白质相互作用的最通用方法之一，并且近年来它已成为一项成熟且可靠的技术。对该技术的进一步改进是能够同时检测和区分不止一种相互作用。这在双诱饵系统中得以实现，该系统已成功用于检测靶向较大蛋白质中特定基序的蛋白质和肽，以促进从文库筛选中获得的一组假定相互作用蛋白质中快速鉴定特定相互作用蛋白，并对特定药物介导的蛋白质-蛋白质相互作用破坏进行评分。
