Lotte C, Patouillet C, Zanini M, Messager A, Roguet R
Advanced Research Laboratories, L'Oréal Life Sciences, Aulnay-sous-Bois, France.
Skin Pharmacol Appl Skin Physiol. 2002;15 Suppl 1:18-30. doi: 10.1159/000066679.
Human reconstructed skin models could be very useful tools to quantify percutaneous permeation and absorption. Before using such models, the reproducibility in the same batch and/or various batches, i.e. the relevance of the results obtained, must be verified. The reproducibility of 3 industrial models--EpiDerm, Episkin and SkinEthic--was tested regarding the permeation and skin absorption of 3 topically applied compounds (with a large range of physicochemical properties): lauric acid, caffeine and mannitol. For all the models, the intrabatch reproducibility was greater than the interbatch reproducibility. According to the batches tested, the larger difference in terms of reproducibility between the 3 models was observed in the case of mannitol, a very poor permeant. In this case, the best reproducibility was observed with EpiDerm and Episkin. Moreover, the rank order of the 3 compounds applied, in terms of permeation and skin absorption, was the same as that expected from ex vivo human skin. Such results revealed human skin models as a promising means to test in vitro permeation and percutaneous absorption of topical products.
人类重建皮肤模型可能是量化经皮渗透和吸收的非常有用的工具。在使用此类模型之前，必须验证同一批次和/或不同批次中的可重复性，即所获得结果的相关性。针对三种局部应用的化合物（具有广泛的物理化学性质）：月桂酸、咖啡因和甘露醇，测试了三种工业模型——EpiDerm、Episkin和SkinEthic——的渗透性和皮肤吸收的可重复性。对于所有模型，批内可重复性大于批间可重复性。根据所测试的批次，在渗透性非常差的甘露醇的情况下，观察到三种模型之间在可重复性方面的差异最大。在这种情况下，EpiDerm和Episkin表现出最佳的可重复性。此外，就渗透和皮肤吸收而言，所应用的三种化合物的排序与离体人皮肤预期的排序相同。这些结果表明，人类皮肤模型是测试局部产品体外渗透和经皮吸收的一种有前景的手段。
