Ang Catharina Y W, Cui Yanyan, Chang Hebron C, Luo Wenhong, Heinze Thomas M, Lin Lawrence J, Mattia Antonia
U.S. Food and Drug Administration, National Center for Toxicological Research, Division of Chemistry, Jefferson, AR 72079, USA.
J AOAC Int. 2002 Nov-Dec;85(6):1360-9.
St. John's wort (Hypericum perforatum L.) preparations, a top-selling botanical dietary supplement used primarily as an antidepressant, has recently been used as an ingredient in some food products sold as functional foods. A rapid extraction technique followed by a liquid chromatographic (LC) method was developed to determine 4 characteristic bioactive compounds (pseudohypericin, hypericin, hyperforin, and adhyperforin) from St. John's wort in dietary supplements and functional foods to which it was added. Solid samples, including dried leaf/flower mixture, dietary supplement capsules, tea bags, puff and snack bar, were extracted with methanol by sonication. Noncarbonated, fruit-flavored drinks were centrifuged and mixed with methanol. Compounds were then determined by isocratic, reversed-phase LC with UV detection at 2 wavelengths and further identified or confirmed by photodiode array spectra and LC/mass spectrometry. Within-laboratory method variations (% RSD) were satisfactory. Very low amounts, if any, of the 4 components were found in drink and puff samples, and none was found in the snack bar. The methods developed provide a useful means for the determination of St. John's wort components in dietary supplements and functional foods.
圣约翰草（贯叶连翘）制剂是一种畅销的植物性膳食补充剂，主要用作抗抑郁药，最近已被用作一些作为功能性食品销售的食品中的成分。开发了一种快速提取技术，随后采用液相色谱（LC）方法，以测定膳食补充剂和添加了圣约翰草的功能性食品中圣约翰草的4种特征性生物活性化合物（假金丝桃素、金丝桃素、贯叶连翘素和去甲贯叶连翘素）。固体样品，包括干叶/花混合物、膳食补充剂胶囊、茶包、泡芙和小吃棒，通过超声处理用甲醇提取。非碳酸水果味饮料经离心后与甲醇混合。然后通过等度反相液相色谱在2个波长下进行紫外检测来测定化合物，并通过光电二极管阵列光谱和液相色谱/质谱进一步鉴定或确认。实验室内方法的变异系数（%RSD）令人满意。在饮料和泡芙样品中发现的这4种成分含量极低，在小吃棒中未发现任何成分。所开发的方法为测定膳食补充剂和功能性食品中的圣约翰草成分提供了一种有用的手段。
