Perez-Guijo V, Muñoz E, Escudero A, Veroz R, Sánchez M, Muñoz-Villanueva M C, González R, Peña J, Collantes-Estevez E
Rheumatology Service, Reina Sofía University Hospital, Cordoba, Spain.
Joint Bone Spine. 2002 Oct;69(5):458-62. doi: 10.1016/s1297-319x(02)00430-x.
To evaluate the relationship between the presence of different HLA-DRB1 genes and predisposition to develop a sporadic form of ankylosing spondylitis (AS) in a demographically well-defined population.
One hundred fifteen selected patients with sporadic (non-familial) forms of AS from six different cities and 748 bone marrow donors as control group. All individuals were typed for HLA-B27 by flow cytometry with monoclonal antibodies and PCR -SSP, as well as for HLA-DRB using the Dynal ELI SSO HLA-DRB Test (Dynal AS, Oslo, Norway). The Inno-Lipa DRB Decoder (Innogenetics NV Zwijndrecht, Belgium), was used for high-resolution HLA-DRB typing.
The presence of the DRB101 antigen in the studied population is significantiy higher in B27 positive healthy individuals (bone marrow donors) than in B27 positive AS patients; also, DRB101 is higher in B27 negative AS patients than 827 negative controls. The frequency of DRB1*03 is higher in B27 negative controls than B27 negative AS patients.
The results of this study suggest that DRB1*01 antigens might be involved in the development of sporadic forms of ankylosing spondylitis in HLA-B27 negative individuals in the studied area.
在一个人口统计学特征明确的人群中，评估不同HLA - DRB1基因的存在与散发性强直性脊柱炎（AS）易感性之间的关系。
从六个不同城市选取115例散发性（非家族性）AS患者作为研究对象，并选取748名骨髓捐献者作为对照组。所有个体均采用单克隆抗体流式细胞术和PCR - SSP法进行HLA - B27分型，同时使用Dynal ELISA SSO HLA - DRB检测试剂盒（挪威奥斯陆Dynal AS公司）进行HLA - DRB分型。采用Inno - Lipa DRB解码器（比利时Zwijndrecht市Innogenetics NV公司）进行HLA - DRB高分辨率分型。
在所研究人群中，DRB101抗原在B27阳性健康个体（骨髓捐献者）中的存在率显著高于B27阳性AS患者；此外，DRB101在B27阴性AS患者中的存在率高于B27阴性对照组。DRB1*03在B27阴性对照组中的频率高于B27阴性AS患者。
本研究结果提示，在研究区域内，DRB1*01抗原可能参与了HLA - B27阴性个体散发性强直性脊柱炎的发病过程。
