Latchman David S
Institute of Child Health, University College London, 30 Guilford Street, London WC1N 1EH, UK.
Curr Gene Ther. 2002 Dec;2(4):415-26. doi: 10.2174/1566523023347698.
Herpes Simplex Virus (HSV) has a number of advantages as a gene delivery vector, particularly for the nervous system. Thus, it naturally establishes asymptomatic latent infections of neuronal cells. Moreover, it is readily grown in culture to high titre and has a large genome so allowing it to be used to deliver multiple or very large genes. Considerable progress has been made in effectively disabling the virus so that it does not damage the cells it infects but can still deliver an inserted gene effectively. In addition, it is now possible to obtain long-term expression of the transgene in the nervous system, using regulatory elements derived from the latency-associated transcript of the virus. As well as its use in the nervous system, the virus has also been used to successfully deliver genes to a variety of other cell types, including peripheral blood mononuclear cells and cardiac myocytes within the intact heart. In particular, its ability to deliver genes effectively to replicating cancer cells and to dendritic cells offers considerable potential for the use of this virus in cancer therapy.
单纯疱疹病毒（HSV）作为一种基因传递载体具有诸多优势，尤其是在神经系统方面。因此，它能自然地在神经元细胞中建立无症状潜伏感染。此外，它易于在培养物中生长至高滴度，并且具有较大的基因组，从而能够用于传递多个或非常大的基因。在有效使病毒失活方面已经取得了相当大的进展，这样它不会损害所感染的细胞，但仍能有效地传递插入的基因。此外，利用源自病毒潜伏相关转录本的调控元件，现在有可能在神经系统中实现转基因的长期表达。除了在神经系统中的应用，该病毒还已成功用于将基因传递到多种其他细胞类型，包括外周血单核细胞和完整心脏内的心肌细胞。特别是，它能够有效地将基因传递到正在复制的癌细胞和树突状细胞，这为该病毒在癌症治疗中的应用提供了相当大的潜力。
