Cullen Paul J, Sprague George F
Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403-1229, USA.
Eukaryot Cell. 2002 Dec;1(6):884-94. doi: 10.1128/EC.1.6.884-894.2002.
A genetic selection in Saccharomyces cerevisiae for mutants that stimulate the mating pathway uncovered a mutant that had a hyperactive pheromone response pathway and also had hyperpolarized growth. Cloning and segregation analysis demonstrated that BUD14 was the affected gene. Disruption of BUD14 in wild-type cells caused mild stimulation of pheromone response pathway reporters, an increase in sensitivity to mating factor, and a hyperelongated shmoo morphology. The bud14 mutant also had hyperfilamentous growth. Consistent with a role in the control of cell polarity, a Bud14p-green fluorescent protein fusion was localized to sites of polarized growth in the cell. Bud14p shared morphogenetic functions with the Ste20p and Bni1p proteins as well as with the type 1 phosphatase Glc7p. The genetic interactions between BUD14 and GLC7 suggested a role for Glc7p in filamentous growth, and Glc7p was found to have a positive function in filamentous growth in yeast.
在酿酒酵母中对刺激交配途径的突变体进行遗传筛选，发现了一个突变体，其信息素反应途径过度活跃，且生长呈超极化状态。克隆和分离分析表明，BUD14是受影响的基因。在野生型细胞中破坏BUD14会导致信息素反应途径报告基因受到轻度刺激，对交配因子的敏感性增加，以及超伸长的shmoo形态。bud14突变体还具有超丝状生长。与在细胞极性控制中的作用一致，Bud14p-绿色荧光蛋白融合体定位于细胞中极化生长的位点。Bud14p与Ste20p和Bni1p蛋白以及1型磷酸酶Glc7p具有共同的形态发生功能。BUD14与GLC7之间的遗传相互作用表明Glc7p在丝状生长中起作用，并且发现Glc7p在酵母的丝状生长中具有正向功能。
