Zimanyi Monika A, Bertram John F, Black M Jane
Department of Anatomy and Cell Biology, P.O. Box 13C, Monash University, 3800 Victoria, Australia.
Pediatr Nephrol. 2002 Dec;17(12):1000-4. doi: 10.1007/s00467-002-0998-8. Epub 2002 Nov 14.
This study investigated the effects of a high-protein diet during pregnancy on nephron endowment and subsequent levels of blood pressure in the offspring. Female WKY rats were fed either a normal (20%, NPD) or a high (54%, HPD) protein diet during pregnancy. Male offspring were paired at birth. At 4 weeks of age, 1 of the pair was randomly chosen for perfusion fixation, and total glomerular number, and thereby nephron number, was estimated using an unbiased stereological technique. The other rat of the pair was allowed to grow to 30 weeks of age, during which time tail cuff systolic blood pressure was monitored twice weekly. There was no effect of the HPD on birth weight (NPD 4.23+/-0.53 g, HPD 4.26+/-0.45 g, mean+/-SD), kidney weight (NPD 0.372+/-0.049 g, HPD 0.337+/-0.090 g), or total nephron number (NPD 27,191+/-3,512, HPD 26,738+/-4,735). Systolic blood pressure at 30 weeks was 170+/-14 mmHg in NPD and 169+/-14 in HPD offspring. These findings show that a HPD during pregnancy did not lead to an increase in birth weight, kidney weight, or nephron endowment, nor did the HPD affect adult blood pressure.
本研究调查了孕期高蛋白饮食对后代肾单位数量及随后血压水平的影响。雌性WKY大鼠在孕期被喂食正常蛋白饮食（20%，NPD）或高蛋白饮食（54%，HPD）。雄性后代在出生时配对。在4周龄时，随机选择配对中的一只进行灌注固定，使用无偏倚的体视学技术估计肾小球总数，从而估算肾单位数量。配对中的另一只大鼠饲养至30周龄，在此期间每周两次监测尾袖收缩压。高蛋白饮食对出生体重（NPD 4.23±0.53 g，HPD 4.26±0.45 g，平均值±标准差）、肾脏重量（NPD 0.372±0.049 g，HPD 0.337±0.090 g）或总肾单位数量（NPD 27,191±3,512，HPD 26,738±4,735）均无影响。30周龄时，NPD后代的收缩压为170±14 mmHg，HPD后代为169±14 mmHg。这些结果表明，孕期高蛋白饮食不会导致出生体重、肾脏重量或肾单位数量增加，也不会影响成年后的血压。
