Bigwarfe Paul M, White Thomas P, Wood Troy D
Department of Chemistry, Natural Sciences Complex, University at Buffalo, The State University of New York, Buffalo, NY 14260-3000, USA.
Rapid Commun Mass Spectrom. 2002;16(24):2266-72. doi: 10.1002/rcm.854.
The increasing tendency to miniaturize analytical techniques has resulted in the widespread use of nanoelectrospray ionization mass spectrometry. A new polyaniline-coated nanoelectrospray emitter has shown increasing promise as a more durable and stable alternative to traditional metal-coated emitters. In this report, the utility of polyaniline-coated nanoelectrospray emitters in the negative ion mode is investigated. Here, oligonucleotides and peptides have been ionized in the negative mode using polyaniline-coated nanoelectrospray emitters. The emitters were found to be durable for at least an hour in the negative ion mode, during which time the signal was stable. The high amount of electrical discharge usually associated with negative ion mode nanoelectrospray was not problematic with the polyaniline-coated emitters. These characteristics make possible the reliable coupling of low-flow separations to negative ion nanoelectrospray without the worry of emitter failure during the course of the experiment.
分析技术日益小型化的趋势导致了纳米电喷雾电离质谱的广泛应用。一种新型的聚苯胺涂层纳米电喷雾发射器作为传统金属涂层发射器更耐用、更稳定的替代品，已展现出越来越大的前景。在本报告中，对聚苯胺涂层纳米电喷雾发射器在负离子模式下的实用性进行了研究。在此，使用聚苯胺涂层纳米电喷雾发射器在负离子模式下对寡核苷酸和肽进行了电离。发现这些发射器在负离子模式下至少可持续一小时，在此期间信号稳定。通常与负离子模式纳米电喷雾相关的大量放电现象在聚苯胺涂层发射器中不存在问题。这些特性使得低流量分离与负离子纳米电喷雾的可靠联用成为可能，而无需担心在实验过程中发射器出现故障。
