[生长激素受体mRNA在肝细胞癌及配对癌旁肝硬化肝组织中的表达]

[Expression of growth hormone receptor mRNA in hepatocellular carcinoma and matched para-cancer cirrhotic liver tissue].

作者信息

Wang Hong-tao, Wang Jie, Ou Qing-jia, Liu Xiao-ping, Chen Shuang

机构信息

Department of Hepato-biliary Surgery, Memorial Hospital, Sun Yat-sen University Guangzhou 510120, P. R. China.

出版信息

Ai Zheng. 2002 Feb;21(2):146-8.

PMID:12479063

Abstract

BACKGROUND & OBJECTIVE: The growth hormone receptor mRNA is abundant in normal liver tissue, but its expression was not studied in detail in the tissue of liver cancer or para-cancer cirrhotic liver. This study was designed to investigate the expression of growth hormone receptor mRNA in hepatocellular carcinoma and para-cancer cirrhotic liver tissue.

METHODS

The expression of growth hormone receptor mRNA was detected in 37 specimens of hepatocellular carcinoma and their para-cancer liver tissue by RT-PCR.

RESULTS

The expression rate of growth hormone receptor mRNA in hepatocellular cancerous tissue (30/37, 81%) was significantly lower than that in cirrhotic liver tissue (32/32, 100%) (P < 0.05). The expression rate in undifferentiated hepatocellular carcinoma (1/4, 20%) decreased further (P < 0.05). The expression of growth hormone receptor mRNA in cirrhotic liver tissue [ratio of integrated Optical Density (riOD) 30.77% +/- 8.24%, n = 32] was significantly lower than that in normal control liver tissue (riOD 44.93% +/- 6.25%, n = 5) (P < 0.05). The expression in severe cirrhotic liver tissue (riOD 21.90% +/- 4.72%, n = 8) decreased further (P < 0.05).

CONCLUSIONS

The down-regulated expression of growth hormone receptor mRNA was related to the pathological stage of hepatocellular cancer and the stage of liver cirrhosis.

摘要

背景与目的

生长激素受体mRNA在正常肝组织中含量丰富，但在肝癌组织或癌旁肝硬化肝组织中的表达尚未进行详细研究。本研究旨在探讨生长激素受体mRNA在肝细胞癌及癌旁肝硬化肝组织中的表达情况。

方法

采用逆转录聚合酶链反应（RT-PCR）检测37例肝细胞癌及其癌旁肝组织中生长激素受体mRNA的表达。

结果

肝细胞癌组织中生长激素受体mRNA的表达率（30/37，81%）显著低于肝硬化肝组织（32/32，100%）（P<0.05）。未分化肝细胞癌中的表达率（1/4，20%）进一步降低（P<0.05）。肝硬化肝组织中生长激素受体mRNA的表达[积分光密度比值（riOD）30.77%±8.24%，n=32]显著低于正常对照肝组织（riOD 44.93%±6.25%，n=5）（P<0.05）。重度肝硬化肝组织中的表达（riOD 21.90%±4.72%，n=8）进一步降低（P<0.05）。