Wang Hongtao, Chen Shuang, Ou Qingjia, Deng Meihai, Liu Xiaoping
Department of Hepato-biliary Surgery, Sun Yat-sen Memorial Hospital, Sun Yat-Sen University of Medical Sciences, Guangzhou 510120, China.
Zhonghua Yi Xue Za Zhi. 2002 Feb 10;82(3):168-71.
To investigate the expression of growth hormone receptor in human cirrhotic livers.
Radio-ligand binding assay and reverse transcription-polymerase chain reaction (RT-PCR) were used to examine the expression of growth hormone receptor and its mRNA in cirrhotic liver tissues near cancer in 32 cases and cirrhotic liver cells near cancer of 6 patients undergoing radical resection of liver cancers. The results were compared with those in 5 specimens of normal liver tissue and 3 specimens of normal liver cells.
The binding capacity of growth hormone receptors in human cirrhotic liver tissues was significant lower than that in normal control [RT, (22.4 +/- 5.0) fmol/mg protein, n = 32 versus (41.3 +/- 5.3) fmol/mg protein, n = 5; P < 0.05] and decreased further along with the aggravation of cirrhosis (P < 0.05). The number of binding sites of growth hormone receptors in human cirrhotic liver cells were significantly lower than that in normal control [Site, (2.40 +/- 0.93) x 10(4)/cell, n = 6 versus (4.68 +/- 1.14) x 10(4)/cell, n = 3; P < 0.05]. The expression of growth hormone receptor mRNA in human cirrhotic liver tissues was significant lower than that in normal control [riOD, 30.8% +/- 8.2%, n = 32 versus 44.93% +/- 6.25%, n = 5; P < 0.05] and decreased further along with the aggregation of cirrhosis (P < 0.05).
The expression of growth hormone receptor in human cirrhotic liver cells is downregulated. The more severe liver cirrhosis the more the downregulation. The decreased expression of growth hormone receptor mRNA may account for the downregulated expression of growth hormone receptor in cirrhotic liver tissues.
研究生长激素受体在人类肝硬化肝脏中的表达情况。
采用放射性配体结合分析法和逆转录-聚合酶链反应（RT-PCR）检测32例肝癌根治术患者癌旁肝硬化肝组织及6例患者癌旁肝硬化肝细胞中生长激素受体及其mRNA的表达，并与5份正常肝组织标本和3份正常肝细胞标本的结果进行比较。
人类肝硬化肝组织中生长激素受体的结合能力显著低于正常对照组[RT，（22.4±5.0）fmol/mg蛋白，n = 32 对比（41.3±5.3）fmol/mg蛋白，n = 5；P < 0.05]，且随着肝硬化程度的加重进一步降低（P < 0.05）。人类肝硬化肝细胞中生长激素受体的结合位点数显著低于正常对照组[位点，（2.40±0.93）×10⁴/细胞，n = 6对比（4.68±1.14）×10⁴/细胞，n = 3；P < 0.05]。人类肝硬化肝组织中生长激素受体mRNA的表达显著低于正常对照组[riOD，30.8%±8.2%，n = 32对比44.93%±6.25%，n = 5；P < 0.05]，且随着肝硬化程度的加重进一步降低（P < 0.05）。
人类肝硬化肝细胞中生长激素受体的表达下调。肝硬化越严重，下调越明显。生长激素受体mRNA表达降低可能是肝硬化肝组织中生长激素受体表达下调的原因。
