巨大芽孢杆菌脂肪分解系统分析及新型I.4亚家族细菌脂肪酶LipA的克隆
Analysis of Bacillus megaterium lipolytic system and cloning of LipA, a novel subfamily I.4 bacterial lipase.
作者信息
Ruiz Cristian, Blanco Ana, Pastor F I Javier, Diaz Pilar
机构信息
Department of Microbiology, Faculty of Biology, University of Barcelona, Av Diagonal 645, 08028 Barcelona, Spain.
出版信息
FEMS Microbiol Lett. 2002 Dec 17;217(2):263-7. doi: 10.1111/j.1574-6968.2002.tb11485.x.
The lipolytic system of Bacillus megaterium 370 was investigated, showing the existence of at least two secreted lipases and a cell-bound esterase. A gene coding for an extracellular lipase was isolated and cloned in Escherichia coli. The cloned enzyme displayed high activity on short to medium chain length (C(4)-C(8)) substrates, and poor activity on C(18) substrates. On the basis of amino acid sequence homology, the cloned lipase was classified into subfamily I.4 of bacterial lipases.
对巨大芽孢杆菌370的脂解系统进行了研究，结果表明至少存在两种分泌型脂肪酶和一种细胞结合酯酶。分离出一个编码细胞外脂肪酶的基因，并将其克隆到大肠杆菌中。克隆的酶对短至中链长度（C(4)-C(8)）的底物具有高活性，而对C(18)底物的活性较差。基于氨基酸序列同源性，克隆的脂肪酶被归类为细菌脂肪酶的I.4亚家族。
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