Adams Catherine E, Broide Ron S, Chen Yiling, Winzer-Serhan Ursula H, Henderson Theodore A, Leslie Frances M, Freedman Robert
VAMC Department of Psychiatry, University of Colorado Health Sciences Center, 4200 East Ninth Avenue, Box C268-71, Denver, CO 80262, USA.
Brain Res Dev Brain Res. 2002 Dec 15;139(2):175-87. doi: 10.1016/s0165-3806(02)00547-3.
The alpha7 nicotinic receptor has been implicated in the regulation of a variety of developmental processes. The goal of the present study was to assess whether the alpha7 receptor might participate in the regulation of hippocampal ontogeny by describing the spatiotemporal development of alpha7 mRNA and alpha-bungarotoxin binding in rat hippocampal formation. Message for the alpha7 receptor was initially observed in the hippocampal neuroepithelium at embryonic day 13 and in the anlage of the hippocampal formation on embryonic day 14. Binding of alpha-bungarotoxin was initially seen on embryonic day 15 in the dorsal portion of the anlage of stratum oriens and stratum radiatum-lacunosum moleculare, but was never observed in the neuroepithelium. Dramatic elevations in both alpha7 mRNA and alpha-bungarotoxin binding were observed in most regions of the hippocampal formation neonatally. The levels of both alpha7 message and protein gradually decreased during the first three postnatal weeks to adult levels in most regions. The lack of alpha-bungarotoxin binding in the neuroepithelium suggests that the alpha7 receptor does not influence neurogenesis. The early appearance and complex, prolonged pattern of development of the alpha7 receptor suggest that it may influence processes as diverse as cell migration, dendritic elaboration and apoptosis during hippocampal maturation.
α7烟碱型受体已被证明与多种发育过程的调节有关。本研究的目的是通过描述大鼠海马结构中α7 mRNA和α-银环蛇毒素结合的时空发育情况，来评估α7受体是否可能参与海马个体发育的调节。在胚胎第13天，首次在海马神经上皮中观察到α7受体的信息，在胚胎第14天，在海马结构的原基中也观察到了。α-银环蛇毒素的结合最初在胚胎第15天出现在原基的背侧部的内嗅层和辐射层-分子层，但在神经上皮中从未观察到。在新生大鼠的海马结构的大多数区域，均观察到α7 mRNA和α-银环蛇毒素结合显著升高。在出生后的前三周，α7信息和蛋白水平在大多数区域逐渐下降至成年水平。神经上皮中缺乏α-银环蛇毒素结合表明α7受体不影响神经发生。α7受体的早期出现以及复杂、延长的发育模式表明，它可能在海马成熟过程中影响多种过程，如细胞迁移、树突形成和细胞凋亡。
