Bertrand H A, Yu B P, Masoro E J
Mech Ageing Dev. 1975 Jan-Feb;4(1):7-17. doi: 10.1016/0047-6374(75)90003-2.
The complex of membranes of which cells are comprised have been considered as likely sites of deterioration underlying the decline on physiologic competence with age. Although this concept is an attractive one on theoreical grounds, few direct experimental data are available on the effect of age on biological membranes. Since the sarcoplasmic reticulum of skeletal muscle has been well characterized and is an easily studied membrane system in regard both to its composition and function, the effect of age on this membrane system was explored. Rats of 1, 2, 3, 6, 12, 24 and 28 months of age were the source of sarcoplasmic reticulum membranes which were characterized by the following measurements: phospholipid/protein weight ratio; phospholipid composition; the activity of the (Ca++ + Mg++) -ATPase and the steady state concentration of its phosphorylated intermediate; the ATP-dependent, oxalatepromoted Ca++ transport activity. Although there were some differences between age groups in these basic parameters of sarcoplasmic reticulum composition and function, the data obtained provide no clear evidence of deterioration of these membranes with age.
构成细胞的膜复合体被认为可能是随着年龄增长生理功能下降背后的退化部位。尽管从理论角度来看这个概念很有吸引力，但关于年龄对生物膜影响的直接实验数据却很少。由于骨骼肌的肌浆网已经得到了充分的表征，并且就其组成和功能而言是一个易于研究的膜系统，因此探讨了年龄对该膜系统的影响。1、2、3、6、12、24和28月龄的大鼠是肌浆网膜的来源，这些膜通过以下测量进行表征：磷脂/蛋白质重量比；磷脂组成；（Ca++ + Mg++）-ATP酶的活性及其磷酸化中间体的稳态浓度；ATP依赖的、草酸盐促进的Ca++转运活性。尽管在这些肌浆网组成和功能的基本参数方面，不同年龄组之间存在一些差异，但所获得的数据并未提供这些膜随着年龄增长而退化的明确证据。
