Zhou Hai-Kuan, Yu Li-Xin, Zheng Shao-Bin, Min Zhi-Lian, Zhu You-Hua
Department of Urology, Nanfang Hospital, First Military Medical University, Guangzhou 510515, China.
Di Yi Jun Yi Da Xue Xue Bao. 2002 Dec;22(12):1093-5.
To investigate the effect of portal venous inoculation of donor splenocytes combined with cyclosporin A (CsA) administration on cardiac allograft survival in mice.
Heterotopic cardiac transplantation between fully allogenic NIH/q and BALB/C strain mice was performed. A modified procedure of neonatal heart-in-ear transplantation, as originally described by Fulmer et al, was adopted. We prepared donor splenocytes from NIH/q or third-party C57BL/6 spleens for BALB/C recipients, which were injected preoperatively via the recipient portal vein or the systemic vein 1 week before the heart-in-ear transplantation. The recipients were subsequently treated with a short course of the immunosuppressive agent, CsA (4 mg/kg starting from 7 d before the operation till 5 d after it).
Portal venous inoculation of donor splenocytes combined with CsA significantly prolonged cardiac graft survival (n=6, P<0.05) that reached 31.00+/-3.23 d, and 2 of the 6 allografts survived for more than 35 d. Donor splenocytes injected via the systemic vein or third-party C57BL/6 mice splenocytes injected via the portal vein did not prolong graft survival (P>0.05). CsA alone or portal venous inoculation of donor-specific splenocytes alone also prolonged graft survival (P<0.05), with mean graft survival time of 18.50+/-2.59 d and 16.11+/-1.97 d respectively.
Combination of portal venous inoculation of donor-specific splenocytes and CsA can prolong murine cardiac allograft survival, which is donor antigen-specific.
探讨经门静脉接种供体脾细胞联合环孢素A（CsA）给药对小鼠心脏同种异体移植存活的影响。
在完全同种异体的NIH/q和BALB/C品系小鼠之间进行异位心脏移植。采用了Fulmer等人最初描述的改良新生儿耳内心脏移植程序。我们从NIH/q或第三方C57BL/6脾脏制备供体脾细胞用于BALB/C受体，在耳内心脏移植前1周经受体门静脉或体静脉术前注射。随后对受体进行短疗程免疫抑制剂CsA治疗（从手术前7天开始至手术后5天，4mg/kg）。
经门静脉接种供体脾细胞联合CsA显著延长了心脏移植物存活时间（n=6，P<0.05），达到31.00±3.23天，6个同种异体移植物中有2个存活超过35天。经体静脉注射供体脾细胞或经门静脉注射第三方C57BL/6小鼠脾细胞均未延长移植物存活时间（P>0.05）。单独使用CsA或单独经门静脉接种供体特异性脾细胞也延长了移植物存活时间（P<0.05），平均移植物存活时间分别为18.50±2.59天和16.11±1.97天。
经门静脉接种供体特异性脾细胞与CsA联合可延长小鼠心脏同种异体移植存活时间，且具有供体抗原特异性。
