Wang Guqi, Burczynski Frank, Hasinoff Brian, Zhong Guangming
Faculty of Pharmacy, University of Manitoba, Winnipeg, Manitoba R3T 2N2, Canada1.
Department of Microbiology and Immunology, University of Texas Health Science Center at San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229, USA2.
Microbiology (Reading). 2002 Dec;148(Pt 12):3955-3959. doi: 10.1099/00221287-148-12-3955.
Chlamydial infection has been associated with myocarditis in animals and humans. However, the mechanism resulting in myocarditis following infection is not known. Here, evidence is presented that both Chlamydia trachomatis and Chlamydia pneumoniae can infect and replicate in myocytes isolated from neonate rats. The infected myocytes contained chlamydial inclusions, indicative of chlamydial growth, and infectious particles were recovered from the infected myocytes. It was also found that chlamydial infection at a late stage induced significant damage to the infected myocytes, as evidenced by an increased lactate dehydrogenase release, reactive oxygen species production and a reduced ATP level. However, no nuclear apoptosis was detected in the infected myocytes. Collectively, these observations have demonstrated that Chlamydia spp. are able to both infect and damage myocytes, suggesting a potential role of chlamydial infection in myocarditis.
衣原体感染在动物和人类中都与心肌炎有关。然而，感染后导致心肌炎的机制尚不清楚。在此，有证据表明沙眼衣原体和肺炎衣原体都能在新生大鼠分离的心肌细胞中感染并复制。被感染的心肌细胞含有衣原体包涵体，这表明衣原体在生长，并且从被感染的心肌细胞中回收了感染性颗粒。还发现衣原体晚期感染对被感染的心肌细胞造成了显著损伤，乳酸脱氢酶释放增加、活性氧产生以及ATP水平降低证明了这一点。然而，在被感染的心肌细胞中未检测到核凋亡。总的来说，这些观察结果表明衣原体能够感染并损伤心肌细胞，提示衣原体感染在心肌炎中可能发挥作用。
