Goodwin J F
Clin Chem. 1976 Feb;22(2):169-72.
In the reaction of m-aminophenol with carbohydrates in a thiourea/acetic acid reagent, addition of borate suppresses chromogen formation for hexoses and pentoses, and enhances it for disaccharides. All reaction products absorb maximally at 520 nm. The relatively greater intensity of the m-aminophenol/maltose reaction product was used in a saccharogenic method for amylase, with low blank readings. This method is compared with two dye/starch procedures.
在间氨基酚与碳水化合物在硫脲/乙酸试剂中的反应中，加入硼酸盐会抑制己糖和戊糖的色原形成，而增强二糖的色原形成。所有反应产物在520nm处有最大吸收。间氨基酚/麦芽糖反应产物相对较强的吸光度被用于淀粉酶的糖化法中，空白读数较低。该方法与两种染料/淀粉法进行了比较。
