Moss D W
Clin Chim Acta. 1976 Mar 1;67(2):169-74. doi: 10.1016/0009-8981(76)90256-4.
Apoenzyme of aspartate aminotransferase in serum can be reactivated conveniently by addition of 100 mumoles/l pyridoxal phosphate to the reaction mixture, without extending the usual minimum pre-incubation period in the operation of the LKB 8600 reaction rate analyzer. Normal sera contain some apoenzyme, the amount of which, as well as that of holoenzyme, is greatly increased by damage to skeletal muscle. This may be due to direct injury or to the indirect effects of anoxia; e.g., following surgery with extracorporeal circulation. Myocardial infarction also increases the levels of both apo- and holoenzymes, but changes in the two levels follow similar time courses and apo- and holo-aminotransferases disappear from the circulation at similar rates.
在反应混合物中加入100微摩尔/升磷酸吡哆醛，可方便地使血清中天冬氨酸转氨酶的脱辅基酶重新激活，而无需延长LKB 8600反应速率分析仪操作中通常的最短预温育时间。正常血清含有一些脱辅基酶，骨骼肌受损会使其含量以及全酶的含量大幅增加。这可能是由于直接损伤或缺氧的间接影响所致；例如，体外循环手术后。心肌梗死也会使脱辅基酶和全酶的水平升高，但两者水平的变化遵循相似的时间进程，脱辅基和全天冬氨酸转氨酶从循环中消失的速率相似。
