Mäkinen K K, Virtanen K K
Clin Chim Acta. 1976 Mar 1;67(2):213-8. doi: 10.1016/0009-8981(76)90262-x.
The activity of arginine aminopeptidases in human serum was studied with special reference to the possible involvement of enzymes resembling aminopeptidase B (APB). The serum samples were obtained from 10 patients with various acute inflammatory processes, both during the acute phase and after treatment with penicillin. The administration of penicillin to the patients did not result in noticable changes in the chromatographic pattern of the APB-like enzyme. The disappearance of the acute symptoms did not significantly affect the overall specific activity of arginine aminopeptidases in serum. The results showed that human serum does contain, contrary to earlier reports, an enzyme corresponding to APB, even though in very low concentrations. The low enzyme activity was revealed by prolonged reaction times and by the use of larger samples in chromatography than earlier. The APB-like enzyme in serum may have derived from blood cells.
对人血清中精氨酸氨肽酶的活性进行了研究，特别关注类似于氨肽酶B（APB）的酶可能的参与情况。血清样本取自10名患有各种急性炎症过程的患者，分别在急性期和青霉素治疗后采集。给患者使用青霉素并未导致APB样酶的色谱图出现明显变化。急性症状的消失对血清中精氨酸氨肽酶的总体比活性没有显著影响。结果表明，与早期报告相反，人血清中确实含有一种与APB相对应的酶，尽管浓度非常低。通过延长反应时间以及在色谱分析中使用比以前更大的样本量，才揭示出这种低酶活性。血清中的APB样酶可能来源于血细胞。
