Kong K F, Yeap S S, Chow S K, Phipps Maude E
Department of Allied Health Sciences, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia.
Autoimmunity. 2002 Jul;35(4):235-9. doi: 10.1080/08916930290024179.
Worldwide population studies have generally agreed that rheumatoid arthritis (RA) is associated with a group of HLA-DRB1 alleles which share a common amino acid sequence at residues 70-74. This represents the first study to investigate the association of HLA-DRB1 genes with susceptibility to RA amongst Malay, Chinese and Indian ethnic groups in Malaysia. One hundred and thirty three RA patients and one hundred and sixty seven healthy controls were recruited. The HLA-DRB1 alleles were studied using the Phototyping method. The subtypes of HLA-DR4 were detected by "high resolution" PCR-SSP DRB104 typing techniques. The prevalence of HLA-DRB10405 was significantly higher in Malay patients with RA than in healthy controls (28.9 vs. 8.3%, p = 0.0016, OR = 4.48, 95% CI = 1.26-16.69). Similarly, DRB10405 was more common in Chinese RA patients than in controls (30.0 vs. 6.7%, p = 0.0029, OR = 6.00, 95% CI = 1.67-23.48). In addition, DRB10901 was a predisposing factor (32.0 vs. 6.7%,p = 0.0015, OR = 6.59, 95% CI = 1.85-25.64) and 0301/04 had a protective role (4.0vs. 25.0%, p = 0.00562, OR = 0.13, 95% CI = 0.02-0.62) in Malaysian Chinese RA. RA in Indians was associated with DRB11001 (51.1 vs. 8.5%,p = 0.00002, OR = 11.24, 95% CI = 3.13-44.18). DRB1*0701 (13.3 vs. 42.6%,p = 0.0022, OR = 2.73, 95% CI = 1.40-5.37) may have a protective effect. Therefore, in the Malaysian population, RA is primarily associated with the QRRAA motif, and we suggest that genetic factors play a crucial role in the pathogenesis of RA, compared to environmental factors.
全球人口研究普遍认为，类风湿性关节炎（RA）与一组HLA - DRB1等位基因相关，这些等位基因在第70 - 74位氨基酸残基处具有共同的氨基酸序列。这是第一项研究马来西亚马来族、华族和印度族人群中HLA - DRB1基因与RA易感性之间关联的研究。招募了133例RA患者和167例健康对照。采用光定型法研究HLA - DRB1等位基因。通过“高分辨率”PCR - SSP DRB104分型技术检测HLA - DR4的亚型。马来西亚马来族RA患者中HLA - DRB10405的患病率显著高于健康对照（28.9%对8.3%，p = 0.0016，OR = 4.48，95% CI = 1.26 - 16.69）。同样，DRB10405在华裔RA患者中比在对照中更常见（30.0%对6.7%，p = 0.0029，OR = 6.00，95% CI = 1.67 - 23.48）。此外，DRB10901是一个易感因素（32.0%对6.7%，p = 0.0015，OR = 6.59，95% CI = 1.85 - 25.64），而0301/04在马来西亚华裔RA中具有保护作用（4.0%对25.0%，p = 0.00562，OR = 0.13，95% CI = 0.02 - 0.62）。印度族的RA与DRB11001相关（51.1%对8.5%，p = 0.00002，OR = 11.24，95% CI = 3.13 - 44.18）。DRB1*0701（13.3%对42.6%，p = 0.0022，OR = 2.73，95% CI = 1.40 - 5.37）可能具有保护作用。因此，在马来西亚人群中，RA主要与QRRAA基序相关，并且我们认为与环境因素相比，遗传因素在RA的发病机制中起关键作用。
