L-精氨酸和抗中性粒细胞血清可实现对高胆固醇血症大鼠毛细血管灌注的微静脉控制。

L-arginine and antineutrophil serum enable venular control of capillary perfusion in hypercholesterolemic rats.

作者信息

Nellore Kavitha, Harris Norman R

机构信息

Bioengineering Department, Pennsylvania State University, University Park, PA, USA.

出版信息

Microcirculation. 2002 Dec;9(6):477-85. doi: 10.1038/sj.mn.7800162.

DOI:10.1038/sj.mn.7800162

PMID:12483545

Abstract

OBJECTIVE

The purpose of this study was to investigate and counteract dysfunctional control of capillary flow in hypercholesterolemia. Capillary flow is controlled by arteriolar tone, which in turn is influenced by mediators released from closely paired venules in a mechanism that involves nitric oxide (NO). However, venular control of capillary flow is altered with hypercholesterolemia.

METHODS

Rats were given a normal or high-cholesterol diet before measurements of mesenteric capillary red blood cell velocity. The arteriolar pathway leading to the capillary was videotaped to measure the percent of the surrounding area (within 15 |gmm) that was occupied by a venule (% pairing).

RESULTS

Venule-paired arterioles were significantly smaller in hypercholesterolemia compared with normocholesterolemia, corresponding to slower capillary flow. A positive correlation between capillary velocity and % pairing observed in normocholesterolemia was not observed during NO synthase inhibition or in hypercholesterolemic rats. However, positive correlations between the two parameters were found in hypercholesterolemia when the rats were given drinking water supplementation of L-arginine or an injection of antineutrophil serum, both of which tended to improve velocity in capillaries branching from venule-paired arteriolar pathways.

CONCLUSIONS

Dysfunctional venular control of capillary perfusion in hypercholesterolemia may be a consequence of a neutrophil-mediated deficiency of NO.

摘要

目的

本研究旨在探究并对抗高胆固醇血症时毛细血管血流的功能失调性控制。毛细血管血流由小动脉张力控制，而小动脉张力又受紧密配对的小静脉释放的介质影响，这一机制涉及一氧化氮（NO）。然而，高胆固醇血症会改变小静脉对毛细血管血流的控制。

方法

在测量肠系膜毛细血管红细胞速度之前，给大鼠喂食正常或高胆固醇饮食。对通向毛细血管的小动脉路径进行录像，以测量被小静脉占据的周围区域（15微米范围内）的百分比（配对百分比）。

结果

与正常胆固醇血症相比，高胆固醇血症时小静脉配对的小动脉明显更小，这与毛细血管血流较慢相对应。在正常胆固醇血症中观察到的毛细血管速度与配对百分比之间的正相关，在一氧化氮合酶抑制期间或高胆固醇血症大鼠中未观察到。然而，当给高胆固醇血症大鼠补充L-精氨酸饮用水或注射抗中性粒细胞血清时，在高胆固醇血症中发现了这两个参数之间的正相关，这两种处理都倾向于改善从小静脉配对的小动脉路径分支的毛细血管中的速度。