应用部分16S rDNA序列作为快速鉴定芽孢杆菌属物种的指标。
Application of the partial 16S rDNA sequence as an index for rapid identification of species in the genus Bacillus.
作者信息
Goto Keiichi, Omura Tomoko, Hara Yukihiko, Sadaie Yoshito
机构信息
Laboratory of QC and Food Analysis, Food Research Institute, Mitsui Norin Co., Ltd., Shizuoka 426-0133, Japan.
出版信息
J Gen Appl Microbiol. 2000 Feb;46(1):1-8. doi: 10.2323/jgam.46.1.
A comparison of 16S rRNA gene (rDNA) sequences was made among type strains of 69 Bacillus species approved in the International Journal of Systematic Bacteriology (IJSB) until 1998. The results suggested that 5' end region (approx. 275 bp) was the hypervariant region (HV region) in the gene and was highly specific for each type strain. Furthermore, a sequence analysis of the HV region of Bacillus strains revealed that this region was highly conserved within the species. These results indicate that the HV region is a useful index for the identification or grouping of Bacillus species.
对截至1998年在《国际系统细菌学杂志》（IJSB）上被认可的69种芽孢杆菌属菌种的模式菌株进行了16S rRNA基因（rDNA）序列比较。结果表明，该基因的5'端区域（约275 bp）是高变区（HV区），对每种模式菌株具有高度特异性。此外，芽孢杆菌菌株HV区的序列分析表明，该区域在种内高度保守。这些结果表明，HV区是芽孢杆菌属菌种鉴定或分组的有用指标。
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