Yasueda Shin-ichi, Kimura Masako, Ohtori Akira, Kakehi Kazuaki
Senju Pharmaceutical Co., Ltd. 2-5-8, Hiranomachi, Chuo-Ku, Osaka 541-0046, Japan.
J Pharm Biomed Anal. 2003 Jan 15;30(6):1735-42. doi: 10.1016/s0731-7085(02)00515-0.
A specific and sensitive method for the determination for difluprednate (DFBA) and its metabolite (deacetylated DFBA, DFB) in aqueous humor was developed. DFBA and DFB were initially absorbed on a Pinkerton-type column, then analyzed by high-performance liquid chromatography using a semi-micro column after column switching. Under the optimized conditions, calibration curves for DFBA and DFB showed good linearity over the range of 1.0-50 and 0.5-50 ng/ml, respectively. We applied the method to the analysis of DFBA and DFB in rabbit aqueous humor, and found that DFBA in rabbit aqueous humor 1 h after instillation of 0.002% DFBA ophthalmic emulsion was not detected, but DFB was present at the concentration of 4.3+/-3.1 ng/ml.
建立了一种测定房水中双氟泼尼酯（DFBA）及其代谢物（脱乙酰双氟泼尼酯，DFB）的特异性灵敏方法。DFBA和DFB首先吸附在平克顿型柱上，然后在柱切换后使用半微柱通过高效液相色谱进行分析。在优化条件下，DFBA和DFB的校准曲线分别在1.0 - 50和0.5 - 50 ng/ml范围内显示出良好的线性。我们将该方法应用于兔房水中DFBA和DFB的分析，发现滴注0.002% DFBA眼用乳剂1小时后，兔房水中未检测到DFBA，但DFB的浓度为4.3±3.1 ng/ml。
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