Maltese Adriana, Maugeri Francesco, Bucolo Claudio
Bausch & Lomb Oftal, Corso Italia 141, 95127 Catania, Italy.
J Chromatogr B Analyt Technol Biomed Life Sci. 2004 May 25;804(2):441-3. doi: 10.1016/j.jchromb.2004.01.058.
A rapid method was developed for quantification of nimesulide (methanesulfonamide, N-[4-nitro-2-phenoxyphenil]) in rabbit aqueous humor. The analyses were performed by high-performance liquid chromatography using a C(18) reversed-phase column (Ultracarb ODS) with UV detection at 300 nm. The mobile phase consisted of acetonitrile-water containing 1% triethylamine (TEA) adjusted to pH 3.2 with orthophosphoric acid. The retention time was 4.5 min. A simple pre-treatment with acetonitrile was used to deproteinize aqueous humor samples. The limit of quantitation was 50 ng/ml. The recovery was over 90%. The relationship between peak areas and concentration was linear over the range between 0.05 and 2.5 microg/ml, with r(2) values over 0.99. The assay provided good reproducibility and accuracy and proved to be suitable for pharmacokinetic studies of nimesulide.
开发了一种快速方法用于定量测定兔眼房水中的尼美舒利（甲磺酰胺，N-[4-硝基-2-苯氧基苯基]）。分析采用高效液相色谱法，使用C(18)反相柱（Ultracarb ODS），在300 nm处进行紫外检测。流动相由含1%三乙胺（TEA）的乙腈-水组成，用正磷酸调节至pH 3.2。保留时间为4.5分钟。采用简单的乙腈预处理对房水样品进行脱蛋白处理。定量限为50 ng/ml。回收率超过90%。在0.05至2.5 μg/ml范围内，峰面积与浓度之间的关系呈线性，r(2)值超过0.99。该测定方法具有良好的重现性和准确性，证明适用于尼美舒利的药代动力学研究。
