Andersen S S
University of California at Berkeley, Department of Molecular and Cellular Biology, 142 Life Sciences Addition #3200, Berkeley, CA 94720-3200, USA.
Methods Cell Sci. 2001;23(4):205-9. doi: 10.1023/a:1016349232389.
The method described here explains a simple protocol for how to prepare dissociated Zebrafish spinal neuron cultures. The neurons grow fast in a simple culture medium and at room temperature. Considering the advantages afforded by the optical transparency of the Zebrafish embryo combined with the powerful molecular perturbation techniques available, this technique has potential to further advance molecular analysis of axon growth and guidance.
这里描述的方法解释了一种简单的方案，用于制备解离的斑马鱼脊髓神经元培养物。这些神经元在简单的培养基中于室温下生长迅速。考虑到斑马鱼胚胎的光学透明性与现有的强大分子扰动技术相结合所带来的优势，该技术有潜力进一步推进轴突生长和导向的分子分析。
