Karavoltsos Manolis, Mourtas Spyros, Gatos Dimitrios, Barlos Kleomenis
Department of Chemistry, University of Patras, Patras, Greece.
J Pept Sci. 2002 Nov;8(11):615-20. doi: 10.1002/psc.421.
Monophthaloyl diamines derived from naturally occurring amino acids were attached through their free amino functions to resins of the trityl type. The phthaloyl groups were removed by hydrazinolysis, and peptide chains were assembled using Fmoc/tBu-amino acids on the liberated amino functions. The peptidyl aminoalkyl amides obtained were cleaved from the resins by mild acidolysis, with the tBu-side chain protection remaining intact.
源自天然氨基酸的单邻苯二甲酰二胺通过其游离氨基官能团连接到三苯甲基型树脂上。通过肼解去除邻苯二甲酰基，并使用Fmoc/tBu-氨基酸在释放的氨基官能团上组装肽链。通过温和酸解从树脂上裂解得到的肽基氨基烷基酰胺，叔丁基侧链保护保持完整。
