Saccà L, Perez G, Pascucci I, Condorelli M
Horm Metab Res. 1976 Jan;8(1):38-41. doi: 10.1055/s-0028-1093689.
The influence of theophylline ethylenediamine (100 mg/kg i.p.) on gluconeogenesis was studied in normal and in adrenodemedullated and reserpinized rats after overnight fasting by measuring the time-course of Alanine-14C incorporation into Glucose-14C. In the normal rat, theophylline produced a moderate hyperglycemia associated with an increased conversion of alanine to glucose at all time intervals. In addition, a marked rise of plasma levels of insulin and glucagon was observed. In sympathetctomized rats, plasma glucose and gluconeogenesis were again enhanced by theophylline, but the pattern of these modifications differed from that of normal rats since the peak values occurred earlier. Subsequently, both parameters rapidly declined reaching values lower than controls at the end of the experiment. Insulin response to theophylline was higher in sympathectomized animals in comparison to normal rats, while glucagon response was approximately of the same magnitude in the two groups. From these findings it was concluded that theophylline is able to stimulate gluconeogenesis from alanine both in the normal and sympathectomized rat. The different pattern of alanine conversion to glucose seems to depend on the different participation of insulin and catecholamines in the two groups.
通过测量丙氨酸 -14C 掺入葡萄糖 -14C 的时间进程，研究了氨茶碱乙二胺（100毫克/千克腹腔注射）对过夜禁食的正常大鼠、肾上腺髓质切除大鼠和利血平化大鼠糖异生的影响。在正常大鼠中，氨茶碱导致中度高血糖，且在所有时间间隔内丙氨酸向葡萄糖的转化率均增加。此外，还观察到胰岛素和胰高血糖素血浆水平显著升高。在交感神经切除的大鼠中，氨茶碱再次提高了血糖和糖异生，但这些变化模式与正常大鼠不同，因为峰值出现得更早。随后，在实验结束时，这两个参数迅速下降，降至低于对照组的值。与正常大鼠相比，交感神经切除动物对氨茶碱的胰岛素反应更高，而两组中胰高血糖素反应的幅度大致相同。从这些发现可以得出结论，氨茶碱能够在正常大鼠和交感神经切除大鼠中刺激丙氨酸的糖异生。丙氨酸向葡萄糖转化的不同模式似乎取决于两组中胰岛素和儿茶酚胺的不同参与情况。
