骨桥蛋白在结直肠癌及肝转移组织中的表达及临床意义
[Expression and clinical significance of osteopontin in colorectal cancer and liver metastatic tissues].
作者信息
Ding Ling, Zheng Shu
机构信息
Cancer Institute, Zhejiang University, Hangzhou 310009, China.
出版信息
Zhonghua Wai Ke Za Zhi. 2002 Oct;40(10):773-5.
OBJECTIVE
To investigate the possible association of osteopontin (OPN) with liver metastasis in colorectal cancer.
METHODS
RNA was quantitated by RT-PCR from colorectal cancer and surrounding normal tissues, also from liver metastatic tissues and normal liver tissues. ISH was utilized to detect OPN mRNA in colorectal cancer and liver metastatic tissues.
RESULTS
In 44 cases, the expressions of OPN mRNA in colorectal cancer tissues were higher than those in the surrounding normal tissues (t = 4.89, P = 0.000). The expression of OPN mRNA in colorectal cancer was as high as 18.2%, but in liver metastasis was 80% (chi(2) = 22.42, P = 0.000). In ISH, OPN mRNA located in the cytoplasm of colorectal cancer cells.
CONCLUSIONS
The expressions of OPN mRNA were the highest in liver metastatic tissues, moderate in colorectal cancer tissues, and lowest in surrounding normal tissues, indicating that OPN is a potential marker for liver metastasis of colorectal cancer.
目的
探讨骨桥蛋白（OPN）与结直肠癌肝转移之间可能存在的关联。
方法
采用逆转录聚合酶链反应（RT-PCR）对结直肠癌组织、癌旁正常组织、肝转移癌组织及正常肝组织中的RNA进行定量分析。利用原位杂交（ISH）检测结直肠癌组织及肝转移癌组织中OPN mRNA的表达情况。
结果
44例患者中，结直肠癌组织中OPN mRNA的表达高于癌旁正常组织（t = 4.89，P = 0.000）。结直肠癌组织中OPN mRNA的表达率为18.2%，而肝转移癌组织中为80%（χ² = 22.42，P = 0.000）。原位杂交显示，OPN mRNA位于结直肠癌细胞的细胞质中。
结论
OPN mRNA在肝转移癌组织中的表达最高，在结直肠癌组织中中等表达，在癌旁正常组织中表达最低，提示OPN是结直肠癌肝转移的潜在标志物。
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