Guo Wenping, Chen Guian, Zhu Cheng, Wang Hongmei
Department of Obstetrics and Gynecology, the Third Hospital, Peking University, Beijing 100083, China.
Zhonghua Fu Chan Ke Za Zhi. 2002 Oct;37(10):604-7.
To study the expression of matrix metalloproteinase (MMP)-2, 9 and tissue inhibitor of metalloproteinase (TIMP)-1, 2 protein in human endometrial carcinoma tissue and its relation to the invasion and metastasis of endometrial carcinoma.
Immunocytochemistry and zymography techniques were used to measure the MMP-2, MMP-9, TIMP-1, TIMP-2 protein levels and activities in endometrial carcinoma tissue of 37 patients and control group composed of 7 normal postmenstrual endometrial samples.
The MMP-2, MMP-9, TIMP-1 and TIMP-2 proteins mainly expressed in endometrial carcinoma cells, glandular cells and endothelial cells. The strongly positive expression proportions of MMP-2, 9 and TIMP-1 proteins in grade III carcinoma cells were respectively 73%, 20% and 67%, which were higher than those in grade II (13%, 0, 27%) and grade I (0) ones (P < 0.05). They were also higher in endometrial carcinoma with deep layer invasion ones (63%, 16%, 68%) and lymph node metastasis ones (4/4, 3/4, 4/4). TIMP-2 did not show different expression with the tumor invasion and metastasis. In endothelial cells of grade III carcinoma tissue the strongly positive expression proportions of MMP-2 (60%) and TIMP-1 (60%) proteins, not MMP-9, were found in higher amount comparing with those in grade II (20%) and grade I (1/7) tumor tissues (P < 0.05). The density of above four proteins were all found very low in the normal postmenopausal endometrial tissues (P < 0.05). By zymography test the active MMP-2 was the main protein form in adenocarcinoma tissue.
MMP-2 and MMP-9 expression levels in adenocarcinoma tissue correlate with the histologic grade and invasion/metastasis trend. Active MMP-2 is the main form in endometrial cancer.
研究基质金属蛋白酶（MMP）-2、9及金属蛋白酶组织抑制剂（TIMP）-1、2蛋白在人子宫内膜癌组织中的表达及其与子宫内膜癌侵袭和转移的关系。
采用免疫细胞化学和酶谱分析技术，检测37例子宫内膜癌患者癌组织及由7例正常绝经后子宫内膜样本组成的对照组中MMP-2、MMP-9、TIMP-1、TIMP-2蛋白水平及活性。
MMP-2、MMP-9、TIMP-1和TIMP-2蛋白主要表达于子宫内膜癌细胞、腺上皮细胞及内皮细胞。MMP-2、9及TIMP-1蛋白在Ⅲ级癌细胞中的强阳性表达率分别为73%、20%和67%，高于Ⅱ级（13%、0、27%）和Ⅰ级（0）（P<0.05）。在深层浸润的子宫内膜癌（63%、16%、68%）及有淋巴结转移的病例（4/4、3/4、4/4）中其表达也较高。TIMP-2的表达与肿瘤侵袭和转移无关。Ⅲ级癌组织内皮细胞中MMP-2（60%）和TIMP-1（60%）蛋白的强阳性表达率高于Ⅱ级（20%）和Ⅰ级（1/7）肿瘤组织（P<0.05），MMP-9则未发现此差异。在正常绝经后子宫内膜组织中上述4种蛋白的表达均很低（P<0.05）。酶谱分析显示，活性MMP-2是腺癌组织中的主要蛋白形式。
腺癌组织中MMP-2和MMP-9的表达水平与组织学分级及侵袭/转移趋势相关。活性MMP-2是子宫内膜癌中的主要形式。
