通过N-羧基酐法合成某些氨酰-AH-琼脂糖凝胶（作者译）

[Synthesis of some aminoacyl-AH-sepharoses via the N-carboxyanhydride method (author's transl)].

作者信息

Peleteiro Rueda de Rivadulla E, Gorka G, Pfaender P

出版信息

Hoppe Seylers Z Physiol Chem. 1976 Jan;357(1):117-9. doi: 10.1515/bchm2.1976.357.1.117.

DOI:10.1515/bchm2.1976.357.1.117

Abstract

(Aminoacyl)n=1-10-AH-Sepharoses can easily be prepared by the N-carboxy anhydride method from amino acid N-carboxy anhydrides and AH-Sepharose 4B at pH 10.2, 0degreesC in aqueous borate buffer. Syntheses of cystinyl-, cysteinyl-, isoleucyl-, leucyl- and phenylalanyl-AH-Sepharoses are described. An improved preparation for cystine bis(N-carboxy anhydride) is presented.

摘要

（氨酰基）n = 1 - 10 - 氨基己基琼脂糖凝胶可通过N - 羧基酸酐法，在pH 10.2、0℃的硼酸盐缓冲水溶液中，由氨基酸N - 羧基酸酐和氨基己基琼脂糖凝胶4B轻松制备。文中描述了胱氨酰、半胱氨酰、异亮氨酰、亮氨酰和苯丙氨酰 - 氨基己基琼脂糖凝胶的合成方法。还介绍了一种改进的胱氨酸双（N - 羧基酸酐）制备方法。