一种制备N6-取代的ATP-琼脂糖凝胶的简便方法。
A facile method for the preparation of N6-substituted ATP-Sepharose.
作者信息
Eckstein F, Goumet M, Wetzel R
出版信息
Nucleic Acids Res. 1975 Oct;2(10):1771-5. doi: 10.1093/nar/2.10.1771.
Abstract
The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.
摘要
描述了将6-甲基磺酰嘌呤核糖5'-三磷酸连接到琼脂糖凝胶4B上作为制备ATP-琼脂糖凝胶的简便方法。将该反应与6-氯嘌呤核糖苷5'-三磷酸以及N(6)-氨基己酰-ATP的偶联反应进行了比较。还描述了一种无需柱色谱纯化的嘌呤核糖苷5'-磷酸的改进合成方法。
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A novel method for phosphorylation of nucleosides to 5'-nucleotides.一种将核苷磷酸化为5'-核苷酸的新方法。
Tetrahedron Lett. 1967 Dec;50:5065-8. doi: 10.1016/s0040-4039(01)89915-9.
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Protein purification by affinity chromatography. Derivatizations of agarose and polyacrylamide beads.通过亲和色谱法进行蛋白质纯化。琼脂糖和聚丙烯酰胺珠粒的衍生化。
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ATP-and dATP-substituted agaroses and the purification of ribonucleotide reductases.ATP和dATP取代的琼脂糖以及核糖核苷酸还原酶的纯化。
Methods Enzymol. 1974;34:253-61. doi: 10.1016/s0076-6879(74)34021-9.
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N6-immobilized 5 -AMP and NAD+: preparations and applications.N6固定化5-腺苷酸和烟酰胺腺嘌呤二核苷酸：制备方法与应用
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