Eckstein F, Goumet M, Wetzel R
Nucleic Acids Res. 1975 Oct;2(10):1771-5. doi: 10.1093/nar/2.10.1771.
The attachment of 6-methylsulfonylpurineribose 5'-triphosphate to AH-Sepharose 4B as a facile method for the preparation of ATP-Sepharose is described. This reaction is compared to the coupling of 6-chloropurineriboside 5'-triphosphate as well as N(6)-aminocaproyl-ATP. An improved synthesis of purineriboside 5'-phosphates which does not require purification by column chromatography is also described.
描述了将6-甲基磺酰嘌呤核糖5'-三磷酸连接到琼脂糖凝胶4B上作为制备ATP-琼脂糖凝胶的简便方法。将该反应与6-氯嘌呤核糖苷5'-三磷酸以及N(6)-氨基己酰-ATP的偶联反应进行了比较。还描述了一种无需柱色谱纯化的嘌呤核糖苷5'-磷酸的改进合成方法。