Uehara N, Yagihashi A, Kondoh K, Tsuji N, Fujita T, Hamada H, Watanabe N
Division of Laboratory Diagnosis1 and Departments of Clinical Laboratory Medicine2, Plastic and Reconstructive Surgery3 and Molecular Medicine4, Sapporo Medical University School of Medicine, South-1, West-16, Chuo-ko, Sapporo 060-8543, Japan.
J Med Microbiol. 2003 Jan;52(Pt 1):41-45. doi: 10.1099/jmm.0.04985-0.
The objective of this study was to understand more of the innate immune response to Helicobacter pylori by determining the expression of human beta-defensin-2 (hBD-2) in various gastric mucosal tissues and MKN45 gastric cancer cells with or without H. pylori. Semi-quantitative TaqMan RT-PCR and immunohistochemistry were carried out. The antimicrobial effects of a transfected hBD-2 gene against H. pylori were also evaluated. The results showed that hBD-2 was expressed in inflamed gastric mucosal tissues with H. pylori infection, but not in the absence of H. pylori infection. Expression was also detected in gastric cancers in patients with H. pylori infection. Expression was induced in the MKN45 gastric cancer cell line by H. pylori in a manner dependent on the abundance of bacteria. hBD-2-transfected 3T3J2-1 cells secreted hBD-2 protein into the culture medium and this protein inhibited growth of H. pylori completely. The results suggest that hBD-2 may be involved in the pathophysiology of H. pylori-induced gastritis.
本研究的目的是通过测定人β-防御素-2（hBD-2）在有或无幽门螺杆菌的各种胃黏膜组织和MKN45胃癌细胞中的表达，进一步了解对幽门螺杆菌的天然免疫反应。进行了半定量TaqMan RT-PCR和免疫组织化学检测。还评估了转染的hBD-2基因对幽门螺杆菌的抗菌作用。结果显示，hBD-2在幽门螺杆菌感染的炎症性胃黏膜组织中表达，但在无幽门螺杆菌感染时不表达。在幽门螺杆菌感染患者的胃癌中也检测到表达。幽门螺杆菌以依赖细菌数量的方式在MKN45胃癌细胞系中诱导表达。转染hBD-2的3T3J2-1细胞将hBD-2蛋白分泌到培养基中，该蛋白完全抑制幽门螺杆菌的生长。结果表明，hBD-2可能参与幽门螺杆菌诱导的胃炎的病理生理过程。
