Lee T-H, Kato H, Chen S-T, Kogure K, Itoyama Y
Department of Neurology, Tohoku University School of Medicine, 1-1 Seiryo-Machi, Aoba-ku, Sendai 980, Japan.
Neuroreport. 2002 Dec 3;13(17):2271-5. doi: 10.1097/00001756-200212030-00020.
We studied the spatial and temporal expression of BDNF immunoreactivity and mRNA in the hippocampal formation after transient forebrain ischemia in gerbils. Our study demonstrated that in the vulnerable CA1 neurons, there was a prolonged expression disparity between BDNF immunoreactivity and mRNA and the BDNF level was reduced, in contrast to the ischemia-resistant dentate gyrus neurons that showed transient expression disparity and maintained the BDNF level. This expression disparity of the neurotrophic factor may be related to delayed neuronal death. Double immunostaining showed that reactive astroglia and microglia could express BDNF, suggesting a possible involvement of these cells in the mechanism of neuronal survival after ischemia.
我们研究了沙土鼠短暂性前脑缺血后海马结构中脑源性神经营养因子（BDNF）免疫反应性和mRNA的时空表达。我们的研究表明，在易损的CA1神经元中，BDNF免疫反应性和mRNA之间存在长期的表达差异，且BDNF水平降低，这与抗缺血的齿状回神经元形成对比，后者表现出短暂的表达差异并维持BDNF水平。这种神经营养因子的表达差异可能与延迟性神经元死亡有关。双重免疫染色显示，反应性星形胶质细胞和小胶质细胞可表达BDNF，提示这些细胞可能参与了缺血后神经元存活的机制。
