Symons D, Moxham B J
Cardiff School of Biosciences, Cardiff University, Biomedical Sciences Building, Museum Avenue, Cardiff, CF10 3US, Wales, United Kingdom.
Connect Tissue Res. 2002;43(2-3):238-44. doi: 10.1080/03008200290000664.
This study quantifies ultrastructural changes within the mesenchyme of the maxillary and medial/lateral nasal processes before and after formation of the intermaxillary segment. At both day 11 and day 13 of development, 4 fetal rat heads were fixed with 3% glutaraldehyde and processed for electron microscopy. Differences between cells within the facial processes were discerned at day 11 of gestation. Significantly more rough endoplasmic reticulum was present in the cells of the maxillary processes than in the lateral nasal processes (p < .05), and a greater number of cell projections was found in the maxillary processes than in the medial nasal processes (p < .05). At day 13, no significant differences were found in the facial processes. Of particular note, a consistent increase in the number of cell projections within all the facial processes was found on day 13 when compared with day 11 (p < .05). This change may be related to intercellular signaling between the mesenchymal cells at the time of onset of major histogenic changes.
本研究量化了上颌突与内侧/外侧鼻突间充质在颌间段形成前后的超微结构变化。在发育的第11天和第13天，将4个胎鼠头部用3%戊二醛固定并进行电子显微镜处理。在妊娠第11天时，面部突起内的细胞存在差异。上颌突细胞中的粗面内质网明显多于外侧鼻突细胞（p < 0.05），且上颌突中的细胞突起数量多于内侧鼻突（p < 0.05）。在第13天时，面部突起中未发现显著差异。特别值得注意的是，与第11天相比，在第13天时所有面部突起中的细胞突起数量持续增加（p < 0.05）。这种变化可能与主要组织发生变化开始时间充质细胞之间的细胞间信号传导有关。
