Shah N
Histochemistry. 1976 Feb 16;46(2):173-5. doi: 10.1007/BF02462742.
The histochemical localization of ascorbic acid in plant tissues with the alcoholic acidic silver nitrate reagent is shown here to be not specific for ascorbic acid, since some of the polyphenolic substances, including flavonoids, which are known to be widely distributed in plant tissues, are also able to reduce the acidic alcoholic silver nitrate reagent at low temperature (0-4 degrees C) and at pH 2 to 2.5 in dark. This method may perhaps be used for animal tissues where flavonoid pigments do not occur in such large quantities as they do in plants. I therefore, come to the inevitable conclusion that the use of alcoholic acidic silver nitrate reagent in localizing ascorbic acid in plant tissues may be highly misleading.
本文显示，用酸性硝酸银酒精试剂对植物组织中的抗坏血酸进行组织化学定位并非抗坏血酸所特有，因为一些多酚类物质，包括已知在植物组织中广泛分布的黄酮类化合物，在低温（0-4摄氏度）、pH值为2至2.5且黑暗的条件下也能还原酸性硝酸银酒精试剂。该方法或许可用于动物组织，因为动物组织中黄酮类色素的含量不像植物中那样高。因此，我不可避免地得出这样的结论：在植物组织中使用酸性硝酸银酒精试剂定位抗坏血酸可能会产生很大的误导。
