散发性结直肠癌中20号染色体杂合性缺失

Loss of heterozygosity of chromosome 20 in sporadic colorectal cancer.

作者信息

Peng Zhihai, Zhou Chongzhi, Zhang Fang, Ling Yun, Tang Huamei, Bai Shaochun, Liu Wanqing, Qiu Guoqiang, He Lin

机构信息

Department of General Surgery, Shanghai No. 1 People's Hospital, 85 Wujing Road, Shanghai 200080, China.

出版信息

Chin Med J (Engl). 2002 Oct;115(10):1529-32.

PMID:12490103

Abstract

OBJECTIVE

To analyze the loss of heterozygosity (LOH) of chromosome 20 in patients with sporadic colorectal cancer to identify additional loci involved in colorectal tumorigenesis.

METHODS

Polymorphic microsatellite markers were analyzed in 83 colorectal cancer patients' tumor and normal DNA by PCR. PCR products were electrophoresed on an 377 DNA sequencer. Genescan 2.1 and Genotype 2.1 software were used in the LOH scanning and analysis. Comparisons between LOH frequency and clinicopathological data were performed by chi(2) test. P < 0.05 was considered statistically significant.

RESULTS

The average LOH frequency in the long arm, short arm and whole chromosome 20 was 21.1%, 26.7% and 22.8%, respectively. Chromosome 20 exhibited relatively high LOH frequency, particularly in the regions of 20p and 20q11.1-q13.1.

CONCLUSION

There is notable genetic instability on chromosome 20 in sporadic colorectal carcinoma patients; that is, mutation on chromosome 20 is closely associated with sporadic colorectal carcinogenesis. Also, there may be tumor suppressor genes related to sporadic colorectal carcinoma near the region 20q11.1-q13.1.

摘要

目的

分析散发性结直肠癌患者20号染色体杂合性缺失（LOH）情况，以确定参与结直肠癌发生的其他基因座。

方法

采用聚合酶链反应（PCR）对83例结直肠癌患者的肿瘤组织和正常组织DNA中的多态性微卫星标记进行分析。PCR产物在377型DNA测序仪上进行电泳。使用Genescan 2.1和Genotype 2.1软件进行LOH扫描和分析。采用卡方检验对LOH频率与临床病理数据进行比较。P < 0.05被认为具有统计学意义。