散发性结直肠癌中1q31.1 - 32.1杂合性缺失的精细定位

Refined mapping of loss of heterozygosity on 1q31.1-32.1 in sporadic colorectal carcinoma.

作者信息

Zhou Chong-Zhi, Qiu Guo-Qiang, Fan Jun-Wei, Wang Xiao-Liang, Tang Hua-Mei, Huang Li, Sun Yu-Hao, Peng Zhi-Hai

机构信息

Department of General Surgery, Shanghai Jiaotong University affiliated First People's Hospital, 85 Wujin Road, Shanghai 200080, China.

出版信息

World J Gastroenterol. 2008 Mar 14;14(10):1582-7. doi: 10.3748/wjg.14.1582.

DOI:10.3748/wjg.14.1582

PMID:18330952

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2693756/

Abstract

AIM

To explore precise deleted regions and screen the candidate tumor suppressor genes related to sporadic colorectal carcinoma.

METHODS

Six markers on 1q31.1-32.1 were chosen. These polymorphic microsatellite markers in 83 colorectal cancer patients tumor and normal DNA were analyzed via PCR. PCR products were electrophoresed on an ABI 377 DNA sequencer. Genescan 3.1 and Genotype 2.1 software were used for Loss of heterozygosity (LOH) scanning and analysis. Comparison between LOH frequency and clinicopathological factors was performed by c2 test.

RESULTS

1q31.1-32.1 exhibited higher LOH frequency in colorectal carcinoma. The average LOH frequency of 1q31.1-32.1 was 23.0%, with the highest frequency of 36.7% (18/49) at D1S2622, and the lowest of 16.4% (11/67) at D1S412, respectively. A minimal region of frequent deletion was located within a 2 cM genomic segment at D1S413-D1S2622 (1q31.3-32.1). There was no significant association between LOH of each marker on 1q31.1-32.1 and the clinicopathological data (patient sex, age, tumor size, growth pattern or Dukes stage), which indicated that on 1q31.1-32.1, LOH was a common phenomenon in all kinds of sporadic colorectal carcinoma.

CONCLUSION

Through our refined deletion mapping, the critical and precise deleted region was located within 2 cM chromosomal segment encompassing 2 loci (D1S413, D1S2622). No significant association was found between LOH and clinicopathologic features in 1q31.1-32.1.

摘要

目的

探索散发性结直肠癌相关的精确缺失区域并筛选候选抑癌基因。

方法

选取位于1q31.1 - 32.1区域的6个标记。通过聚合酶链反应（PCR）分析83例结直肠癌患者肿瘤组织及正常组织DNA中的这些多态性微卫星标记。PCR产物在ABI 377 DNA测序仪上进行电泳。使用Genescan 3.1和Genotype 2.1软件进行杂合性缺失（LOH）扫描与分析。采用卡方检验对LOH频率与临床病理因素进行比较。

结果

1q31.1 - 32.1在结直肠癌中表现出较高的LOH频率。1q31.1 - 32.1的平均LOH频率为23.0%，其中D1S2622位点的频率最高，为36.7%（18/49），D1S412位点的频率最低，为16.4%（11/67）。频繁缺失的最小区域位于D1S413 - D1S2622（1q31.3 - 32.1）的一个2厘摩（cM）基因组片段内。1q31.1 - 32.1上各标记的LOH与临床病理数据（患者性别、年龄、肿瘤大小、生长方式或杜克分期）之间无显著相关性，这表明在1q31.1 - 32.1上，LOH是各类散发性结直肠癌中的常见现象。